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AB3849-I Anti-phospho SMAD2 Antibody (Ser465/467)

Anti-phospho SMAD2 Antibody (Ser465/467) is an antibody against phospho SMAD2 for use in Western Blotting.

Anti-phospho SMAD2 Antibody (Ser465/467) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: SMAD2, MADH2, MADR2 UniProt Number: Q15796
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AB3849-I
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3849-I
      ReplacesAB3849
      DescriptionAnti-phospho SMAD2 Antibody (Ser465/467)
      Alternate Names
      • Mothers against decapentaplegic homolog 2, Ser465/467 phosphorylated
      • hMAD-2, Ser465/467 phosphorylated
      • hSMAD2, Ser465/467 phosphorylated
      • JV18-1, Ser465/467 phosphorylated
      • MAD homolog 2, Ser465/467 phosphorylated
      • Mad-related protein 2, Ser465/467 phosphorylated
      • Mothers against DPP homolog 2, Ser465/467 phosphorylated
      • SMAD 2, Ser465/467 phosphorylated
      • SMAD family member 2, Ser465/467 phosphorylated
      • Smad2, Ser465/467 phosphorylated
      Background InformationMothers against decapentaplegic homolog 2 (UniProt Q15796; also known as hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, Smad2) is encoded by the SMAD2 (also known as MADH2, MADR2) gene (Gene ID 4087) in human. Smad2 is a receptor-regulated intracellular signal transducer and transcriptional modulator activated by transforming growth factor TGF-beta and activin type 1 receptor kinases. In response to TGF-beta signal, smad2 is recruited to the activated TGF-beta receptor through its interaction with the SMAD anchor for receptor activation (SARA) protein, where it is phosphorylated by the TGF-beta receptor. The phosphorylation results in its dissociation from SARA and association with another family member smad4. The smad2-smad4 complex then translocates into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Smad2 can also be phosphorylated by activin type 1 receptor kinase, and thereby transduce activin-dependent signaling events.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho SMAD2 Antibody (Ser465/467) is an antibody against phospho SMAD2 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.
      EpitopePhospho-Ser465/467.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody detects Smad2 Ser465/467 phosphorylation in TGFbeta-stimulated, but not unstimulated HepG2 cells. The corresponding target phosphoserine residues in the alternatively spliced human Smad2Deltaexon3 (Isoform Short) are pSer435 & pSer437 (UniProt Q15796-2).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Bovine, Xenopus, Zebrafish, Chicken based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMAD2
      • MADH2
      • MADR2
      Modifications
      • Phosphorylation
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~53 kDa. 52.31 kDa (Isoform Long) and 48.96 kDa (Isoform Short; Smad2Deltaexon3) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected Smad2 Ser465/467 phosphorylation in 10 µg lysate from TGFbeta-stimulated, but not unstimulated, HepG2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB3849-I 04055977333800

      Documentation

      Anti-phospho SMAD2 Antibody (Ser465/467) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho SMAD2 Antibody (Ser465/467) Certificates of Analysis

      TitleLot Number
      Anti-phospho SMAD2 (Ser465/467) - 3172383 3172383
      Anti-phospho SMAD2 (Ser465/467) - 3233096 3233096
      Anti-phospho SMAD2 (Ser465/467) - 3384842 3384842
      Anti-phospho SMAD2 (Ser465/467) - 3451051 3451051
      Anti-phospho SMAD2 (Ser465/467) - 3532294 3532294
      Anti-phospho SMAD2 (Ser465/467) - 3753293 3753293
      Anti-phospho SMAD2 (Ser465/467) - 3910141 3910141
      Anti-phospho SMAD2 (Ser465/467) - 3993464 3993464
      Anti-phospho SMAD2 (Ser465/467) - 4140613 4140613
      Anti-phospho SMAD2 (Ser465/467) -2730005 2730005
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies