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07-892 Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody

This Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody is validated for use in WB, IC for the detection of phospho-Pyk2/CAKβ/FAK2 (Tyr402).

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Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PTK2B, RAFTK, FAK2, PYK2, FADK2, PTK, CADTK, CAKB, PKB UniProt Number: Q14289
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07-892
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-892
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody
      Alternate Names
      • PTK2B
      • Proline-rich tyrosine kinase 2
      • Focal adhesion kinase 2
      • FADK2
      • Cell adhesion kinase beta
      • RAFTK
      • Calcium-dependent tyrosine kinase
      • CADTK
      Product Information
      FormatAffinity Purified
      Presentation100 μl of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody is validated for use in WB, IC for the detection of phospho-Pyk2/CAKβ/FAK2 (Tyr402).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Pyk2 (Tyr402) in RIPA lysate of Pyk2 transfected CEF cells
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human and rat phospho-Pyk2/CAKβ/FAK2 (Tyr402).
      HostRabbit
      SpecificityPyk2 phosphorylated on tyrosine residue 402
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
      Gene Symbol
      • PTK2B
      • RAFTK
      • FAK2
      • PYK2
      • FADK2
      • PTK
      • CADTK
      • CAKB
      • PKB
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14289 # Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.
      SIZE: 1009 amino acids; 115875 Da
      SUBUNIT: Interacts with Crk-associated substrate (Cas), PTPNS1 and SH2D3C (By similarity). Interacts with nephrocystin, DDEF2, OPHN1L, SKAP2 and TGFB1I1.
      SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Interaction with nephrocystin induces the membrane-association of the kinase.
      TISSUE SPECIFICITY: Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
      PTM: Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha- lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity (By similarity).
      SIMILARITY: SwissProt: Q14289 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
      Molecular Weight115 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      07-892 04053252731129