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OP29L Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)

This Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) is validated for use in FC, Frozen Sections, Gel Shift, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-3) (Mutant).

Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP29L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ch, Ht, H, M, RMMonoclonal Antibody

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OP29L-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~53 kDa mutant p53 protein under non-denaturing conditions by immunoprecipitation, immunofluorescence, and flow cytometry. Recognizes both mutant and wild-type p53 by immunoblotting and paraffin sections under denaturing conditions.
      Catalogue NumberOP29L
      Brand Family Calbiochem®
      References
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlSK-OV-3 cells
      Positive controlA431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections Pezzella, F., et al. 1994. J. Clin. Pathol. 47, 592. Original Clone Gannon, J.V., et al. 1990. EMBO J. 9, 1595. Epitope Identification Stephen, C.W. and Lane, D.P., 1992. J. Mol. Biol. 225, 1. Immunofluorescence Bernardi, R., et al. 2004. Nat. Cell Biol. 6, 665.
      Key Applications Flow Cytometry
      Frozen Sections
      Gel Shift
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesFlow Cytometry (1-20 µg/ml)
      Frozen Sections (10 µg/ml)
      Gel Shift (see comments)
      Immunoblotting (5 µg/ml)
      Immunofluorescence (1-20 µg/ml)
      Immunoprecipitation (1 µg per sample)
      Paraffin Sections (see application references)
      Application CommentsUnder non-denaturing conditions (immunoprecipitation, immunofluorescence and frozen sections), p53 (Ab-3) does not bind to normal (wild-type) p53 protein; it recognizes an epitope exposed by activating mutations or denaturation. In denaturing protocols (immunoblotting and paraffin sections), p53 (Ab-3) will recognize both mutant and wild-type p53. Will not recognize to some p53 molecules with mutations in the RHSVV epitope, but will recognize TFIIIA, which has the RHSVV epitope. For gel shift assay, resuspend in 100 µl buffer. Antibodies should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase
      ImmunogenHuman
      Epitopewithin amino acids 213-217
      ClonePAb240
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Bovine
      • Chicken
      • Hamster
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP29L-100UG 04055977208405

      Documentation

      Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Certificates of Analysis

      TitleLot Number
      OP29L

      References

      Reference overview
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-February-2008 JSW
      ApplicationFlow Cytometry (1-20 µg/ml)
      Frozen Sections (10 µg/ml)
      Gel Shift (see comments)
      Immunoblotting (5 µg/ml)
      Immunofluorescence (1-20 µg/ml)
      Immunoprecipitation (1 µg per sample)
      Paraffin Sections (see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing of splenocytes with SP2 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions and both mutant and wild-type p53 under denaturing conditions.
      BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase
      Epitopewithin amino acids 213-217
      ClonePAb240
      IsotypeIgG₁
      Speciesnot Xenopus, bovine, chicken, hamster, human, mouse, rat
      Positive controlA431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma
      Negative controlSK-OV-3 cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsUnder non-denaturing conditions (immunoprecipitation, immunofluorescence and frozen sections), p53 (Ab-3) does not bind to normal (wild-type) p53 protein; it recognizes an epitope exposed by activating mutations or denaturation. In denaturing protocols (immunoblotting and paraffin sections), p53 (Ab-3) will recognize both mutant and wild-type p53. Will not recognize to some p53 molecules with mutations in the RHSVV epitope, but will recognize TFIIIA, which has the RHSVV epitope. For gel shift assay, resuspend in 100 µl buffer. Antibodies should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide
      Toxicity Standard Handling
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
      Application referencesParaffin Sections Pezzella, F., et al. 1994. J. Clin. Pathol. 47, 592. Original Clone Gannon, J.V., et al. 1990. EMBO J. 9, 1595. Epitope Identification Stephen, C.W. and Lane, D.P., 1992. J. Mol. Biol. 225, 1. Immunofluorescence Bernardi, R., et al. 2004. Nat. Cell Biol. 6, 665.

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      Catalogue Number Description  
      OP29 Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies