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36-011-I-100UL Anti-nitro-α/β-Synuclein Antibody, clone nSyn12

Anti-nitro-a/β-Synuclein, clone nSyn12, Cat. No. 36-011-I, is a mouse monoclonal antibody that detects nitro-a/β-Synuclein and is is tested for use in Immunohistochemistry (Paraffin).

36-011-I-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue Number36-011-I-100UL
      DescriptionAnti-nitro-α/β-Synuclein Antibody, clone nSyn12
      Alternate Names
      • Non-A beta component of AD amyloid
      • Non-A4 component of amyloid precursor
      • NACP
      • SNCB
      Background Informationa-synuclein (UniProt: P37840; also known Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) and beta-synuclein (UniProt: Q16143) are encoded by the SNCA (also known as NACP, PARK1) and SNCB genes, respectively in human. a-synuclein is abundantly expressed in the brain and is found in a classic amyloid fibril form within the intra-neuronal Lewy body deposits of Parkinson's disease brains. In its monomeric form it participates in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. It acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. In its multimeric form it acts as a molecular chaperone assisting in the folding of synaptic fusion components called SNAREs at presynaptic membrane. It is also involved in the regulation of dopamine release and transport. It is also reported to reduce neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Post-translationally synucleins can be nitrosylated. Higher levels of nitrosylation has been reported in a-synucleinopathies, such as in Parkinson's disease (PD), where 3-nitrotyrosinated a-synuclein is detected in brain aggregates. The intensity of nitrosylation of tyrosine 125 and 136 is shown to be enhanced and that of the tyrosine 39 is reduced in PD patients. a-synuclein can also undergo phosphorylation on serine residues. Phosphorylation of serine 129 is reported to be selective and extensive in synucleinopathy lesions. Genetic alterations of SNCA gene is reported to result in aberrant polymerization into fibrils, which is associated with several neurodegenerative diseases (synucleinopathies). Mutations in SNCA gene are also known to Parkinson's disease characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology of Parkinson s disease involves progressive loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies in surviving neurons in various areas of the brain. Clone nSyn12 detects a-Synuclein nitrated at Tyr125/Tyr136 and β-Synuclein nitrated at Tyr130. (Ref.: Fernandez, E., et al. (2013). Antitoxic. Redox Signal. 19(9); 912-918).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-nitro-a/β-Synuclein, clone nSyn12, Cat. No. 36-011-I, is a mouse monoclonal antibody that detects nitro-a/β-Synuclein and is is tested for use in Immunohistochemistry (Paraffin).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenFull-length human recombinant Synuclein nitrated in vitro.
      Epitopea-Synuclein nitrated at Tyr125 / Tyr136 and β-Synuclein nitrated at Tyr130
      ClonenSyn12
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone nSyn12 is a mouse monoclonal antibody that detects nitrated human a and β-synuclein.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SNCA
      • NACP
      • PARK1
      • SNCB
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight14.46 kDa and 14.29 kDa, respectively for a-Synuclein and β-Synuclein.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human Parkinson's diseased brain tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected nitro-a/β-Synuclein in human Parkinson's diseased brain tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      36-011-I-100UL 04061842414857