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MABN788-25UG Anti-nitrated Hsp90 Antibody, clone 44.23

Anti-Nitrated Hsp90, clone 44.23, Cat. No. MABN788, is a highly specific mouse monoclonal antibody that targets nitrated HSP90 and has been tested in Dot Blot, ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-nitrated Hsp90 Antibody, clone 44.23 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Hsp90ab1, Hsp84, Hspcb UniProt Number: P34058
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MABN788-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RDB, ELISA, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN788-25UG
      DescriptionAnti-nitrated Hsp90 Antibody, clone 44.23
      Alternate Names
      • Heat shock protein HSP 90-beta
      • Heat shock 84 kDa
      • HSP 84
      • HSP84
      Background InformationHeat shock protein HSP 90-beta (UniProt: P34058; also known as Heat shock 84 kDa, HSP 84, HSP84) is encoded by the Hsp90ab1 (also known as Hsp84, Hspcb) gene (Gene ID: 301252) in rat. HSP90 is a ubiquitous chaperone protein with multiple functions in the cell. It promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. HSP90 undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, such as transcription factors, hormone receptors, and protein kinases, thereby causing their activation. HSP90 is abundant protein in the cell and may account for up to 2% of the total cytosolic protein. HSP90 is considered to be absolutely essential for cell survival. Its anti-apoptotic activity involves the inhibition of Apaf-1 oligomerization and caspase activation. Apart from its chaperone activity, HSP90 also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription by altering the steady-state levels of certain transcription factors in response to various physiological cues and also modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases. HSP90 can also participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Nitration of HSP90 results in decreased chaperone activity and intracellular release of the nitrated HSP90 is shown to induce induces death of PC12 cells. (Ref.: Ye, Y et al (2007). J. Biol. Chem. 282(9); 6324-6337).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Nitrated Hsp90, clone 44.23, Cat. No. MABN788, is a highly specific mouse monoclonal antibody that targets nitrated HSP90 and has been tested in Dot Blot, ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Dot Blot
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected nitrated HSP90 in Western Blotting applications (Ye, Y., et. al. (2007). J Biol Chem. 282(9):6324-37).
      ELISA Analysis: A representative lot detected nitrated HSP90 in ELISA applications (Ye, Y., et. al. (2007). J Biol Chem. 282(9):6324-37).
      Dot Blot Analysis: A representative lot detected nitrated HSP90 in Dot Blot applications (Franco, M.C., et. al. (2013). Proc Natl Acad Sci U S A. 110(12):E1102-11). Immunohistochemistry Analysis: A representative lot detected nitrated HSP90 in Immunohistochemistry applications (Franco, M.C., et. al. (2013). Proc Natl Acad Sci U S A. 110(12):E1102-11).
      Biological Information
      ImmunogenPurified, nitrated HSP90 from rat liver.
      Clone44.23
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 44.23 detects nitrated HSP90 in rat, but not HSP90. It specifically targets nitrotyrosine 56.
      IsotypeIgG1
      Species Reactivity
      • Rat
      Species Reactivity NoteRat.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Hsp90ab1
      • Hsp84
      • Hspcb
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight90 kDa observed; 83.28 kda calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC12 cells treated with pyroxynitrite.
      Western Blotting Analysis:  0.25 µg/mL of this antibody detected nitrated HSP90 in PC12 cells treated with pyroxynitrite.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN788-25UG 04054839522123