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PC05L Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

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PC05L
  
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      Overview

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.
      Catalogue NumberPC05L
      Brand Family Calbiochem®
      References
      ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
      Rouscher III, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
      Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
      Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
      Verma, I. 1986. Trends Genet. 2, 93.
      Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433
      Product Information
      FormLyophilized
      FormulationLyophilized from PBS, a volatile buffer, 100 µg BSA.
      Positive controlHep G2 or NIH 3T3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesGel Shift
      Guo, R-F., et al. 2002. Am. J. Pathol. 161, 275.
      Key Applications Frozen Sections
      Gel Shift
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Not Free-floating Sections
      Paraffin Sections
      Application NotesFrozen Sections (5-10 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg per reaction)
      Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)
      Gel Shift (see comments and application references)
      Free-floating Sections (not recommended)
      Application CommentsFor gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.
      Global Trade Item Number
      Catalogue Number GTIN
      PC05L 0