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MABT825 Anti-beta-Actin Antibody, clone 4C2

This Anti-beta-Actin Antibody, clone 4C2 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.

Anti-beta-Actin Antibody, clone 4C2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: ACTB UniProt Number: P60709
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MABT825
100 μg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Ch, RWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT825
      DescriptionAnti-beta-Actin Antibody, clone 4C2
      Alternate Names
      • Actin, cytoplasmic 1
      • beta-Actin
      Background InformationActin, cytoplasmic 1 (UniProt P60709; also known as Beta-actin) is encoded by the ACTB gene (Gene ID 60) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-beta-Actin Antibody, clone 4C2 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected beta-Actin in 10 µg of NIH/3T3 cell lysate.
      Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot detected beta-Actin in HUVECs, A431 and HeLa cells.
      Western Blotting Analysis: A representative lot detected downregulated beta-actin levels in stimulated (by A23187, TRAP-6, TNF, LPS, or IFN-γ) human cerebral microvascular endothelial D3 cells (hCMEC/D3) and their microparticles (MPs) when compared with unstimulated hCMEC/D3 and their MPs (Latham, S.L., et al. (2013). FASEB J. 27(2):672-683).
      Western Blotting Analysis: A representative lot detected siRNA-mediated downregulation of beta-actin in A549 human lung carcinoma cells (Miazza, V., et al. (2011). Virology. 410(1):7-16).
      Western Blotting Analysis: A representative lot detected beta-actin, but not cytoplasmic gamma-actin separated by 2-D gel electrophoresis of purified chicken gizzard actins or total protein extracts from human subcutaneous fibroblasts (HSCFs), canine MDCK cells, and rat aorta tissue (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).
      Western Blotting Analysis: A representative lot detected BSA conjugated with beta-actin N-terminal peptide, but not BSA conjugated with N-terminal peptides derived from the other 5 actin types (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).
      Immunocytochemistry Analysis: A representative lot detected TNF-stimulated localization of β-actin into thick, intensely staining stress fibers prominent at the basal surface of of human cerebral microvascular endothelial D3 cells (hCMEC/D3). Rho kinase inhibitor Y-27632 (Cat. No. 688000) treatment suppressed TNF-induced β-actin stress fiber formation (Latham, S.L., et al. (2013). FASEB J. 27(2):672-683).
      Immunocytochemistry Analysis: A representative lot detected a drastic subcellular redistribution of beta-actin following Sendai virus infection of polarized Madin-Darby canine kidney (MDCK) epithelial cells by fluorescent immunocytochemistry staining of paraformaldehyde-fixed, methanol-treated cells (Miazza, V., et al. (2011). Virology. 410(1):7-16).
      Immunocytochemistry Analysis: A representative lot detected beta-actin subcellular localization distinct from that of cytoplasmic gamma-actin in both spreading and stationary cells by fluorescent immunocytochemistry, using paraformaldehyde-fixed, methanol-treated HSCF human subcutaneous fibroblasts, HaCaT human keratinocytes, WI38 human embryonic fibroblasts,and Madin-Darby canine kidney (MDCK) cells (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminal sequence of human beta-Actin.
      EpitopeNear N-terminus.
      Clone4C2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4C2 detected BSA conjugated with beta-actin N-terminal peptide, but not BSA conjugated with N-terminal peptides derived from the 5 other actin types (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Chicken
      • Rat
      Species Reactivity NoteHuman, Mouse, Chicken, Rat. Predicted to react with all Mammals based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACTB
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~39-45 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected beta-Actin in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT825 04055977331172

      Documentation

      Anti-beta-Actin Antibody, clone 4C2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-beta-Actin Antibody, clone 4C2 Certificates of Analysis

      TitleLot Number
      Anti-beta-Actin, clone 4C2 - 3243451 3243451
      Anti-beta-Actin, clone 4C2 - 3419587 3419587
      Anti-beta-Actin, clone 4C2 - 3587997 3587997
      Anti-beta-Actin, clone 4C2 - 3701824 3701824
      Anti-beta-Actin, clone 4C2 - 3832694 3832694
      Anti-beta-Actin, clone 4C2 - 3980733 3980733
      Anti-beta-Actin, clone 4C2 - 4111217 4111217
      Anti-beta-Actin, clone 4C2 - 4183415 4183415
      Anti-beta-Actin, clone 4C2 -2772513 2772513
      Anti-beta-Actin, clone 4C2 -2826413 2826413
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies