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MABT823 Anti-alpha-Actin Antibody, Cardiac Muscle Antibody, clone 22D3

This mouse monoclonal Anti-alpha-Actin Antibody, Cardiac Muscle, clone 22D3, Cat. No. MABT823 is validated for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Western Blotting for the detection of alpha-cardiac actin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: ACTC1, ACTC, ASD5, CMD1R, CMH11, LVNC4 UniProt Number: P68032
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MABT823
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Gt, R, Rb, HFC, ICC, IF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT823
      DescriptionAnti-alpha-Actin Antibody, Cardiac Muscle Antibody, clone 22D3
      Alternate Names
      • Actin
      • alpha cardiac muscle 1
      • Alpha-cardiac actin
      Background InformationActin, alpha cardiac muscle 1 (UniProt P68032; also known as Alpha-cardiac actin) is encoded by the ACTC1 (also known as ACTC, ASD5, CMD1R, CMH11, LVNC4) gene (Gene ID 70) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic - and -actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-alpha-Actin Antibody, Cardiac Muscle, clone 22D3, Cat. No. MABT823 is validated for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Western Blotting for the detection of alpha-cardiac actin.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed, 0.05% NP-40-permeabilzed rectangular rabbit cardiomyocytes by indirect immuoflourescence (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).

      Immunofluorescence Analysis: A representative lot immunostained cardiomyocytes in human left ventricular endocardial biopsies, rabbit left ventricular myocardium, as well as in goat atrial myocardium frozen tissue sections by fluorescent immunohistochemistry (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).

      Western Blotting Analysis: A representative lot specifically detected alpha-cardiac actin in rat myocardium homogenate with no cross-reactivity toward - or -smooth muscle actin in rat aorta and chicken gizzard homogenates, cytoplasmic - or -actin in human platelet lysate, nor -skeletal actin in rat skeletal muscle homogenate (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human mature alpha-cardiac actin N-terminal end sequence with an acetylated N-terminus.
      Clone22D3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 22D3 specifically detected alpha-cardiac actin in rat myocardium homogenates with no cross-reactivity toward - or -smooth muscle actin in rat aorta and chicken gizzard homogenates, cytoplasmic - or -actin in human platelet lysate, nor -skeletal actin in rat skeletal muscle homogenate (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).
      IsotypeIgG1κ
      Species Reactivity
      • Goat
      • Rat
      • Rabbit
      • Human
      Species Reactivity NoteHuman, Goat, Rabbit, Rat. Predicted to react with Mouse, Porcine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACTC1
      • ACTC
      • ASD5
      • CMD1R
      • CMH11
      • LVNC4
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight41.78/42.02 kDa (mature/pro-form) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HeLa cells.

      Flow Cytometry Analysis: 2 µL of this antibody detected alpha-cardiac actin in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilzed HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT823 04054839056345