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MABE1819-25UG Anti-XPD Antibody, clone 22TF2-2F6

Anti-XPD, clone 22TF2-2F6, Cat. No. MABE1819, is a highly specific mouse monoclonal antibody that targets human General transcription and DNA repair factor IIH helicase subunit XPD and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-XPD Antibody, clone 22TF2-2F6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: ERCC2, XPD, XPDC UniProt Number: P18074
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MABE1819-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1819-25UG
      DescriptionAnti-XPD Antibody, clone 22TF2-2F6
      Alternate Names
      • General transcription and DNA repair factor IIH helicase subunit XPD
      • EC: 3.6.4.12
      • TFIIH subunit XPD
      • Basic transcription factor 2 80 kDa subunit
      • BTF2 p80
      • CXPD
      • DNA excision repair protein ERCC-2
      • DNA repair protein complementing XP-D cells
      • TFIIH basal transcription factor complex 80 kDa subunit
      • TFIIH 80 kDa subunit
      • TFIIH p80
      • Xeroderma pigmentosum group D-complementing protein
      Background InformationGeneral transcription and DNA repair factor IIH helicase subunit XPD (UniProt: P18074; also known as EC: 3.6.4.12, TFIIH subunit XPD, Basic transcription factor 2 80 kDa subunit, BTF2 p80, CXPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells, TFIIH basal transcription factor complex 80 kDa subunit, TFIIH 80 kDa subunit, TFIIH p80, Xeroderma pigmentosum group D-complementing protein) is encoded by the ERCC2 (also known as XPD, XPDC) gene (Gene ID: 2068) in human. XPD is an ATP-dependent 5'-3' DNA helicase component of the general transcription and DNA repair factor IIH (TFIIH) core complex that is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA. Its ATP-binding region is localized to amino acids 7-283 and its nuclear localization is in amino acids 682-695. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH plays an essential role in transcription initiation. When the pre-initiation complex has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail of the largest subunit of RNA polymerase II by CDK-activating kinase (CAK) complex controls the initiation of transcription. XPD acts by forming a bridge between CAK and the core-TFIIH complex. Mutations in ERCC2 gene are known to cause Xeroderma pigmentosum complementation group D that is characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. The skin develops marked freckling and other pigmentation abnormalities.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-XPD, clone 22TF2-2F6, Cat. No. MABE1819, is a highly specific mouse monoclonal antibody that targets human General transcription and DNA repair factor IIH helicase subunit XPD and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected XPD in Western Blotting applications (Alekseev, S., et. al. (2017). Mol Cell. 65(3):504-514.e4).

      Immunocytochemistry Analysis: A representative lot detected XPD in Immunocytochmeistry applications (Vermeulen, W., et. al. (2000). Nat Genet. 26(3):307-13). .

      Immunoprecipitation Analysis: A representative lot immunoprecipitated XPD in Immunoprecipitation applications (Coin, F., et. al. (1998). Nat Genet. 20(2):184-8; Seroz, T., et. al. (2000). Nucleic Acids Res. 28(22):4506-13).
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to 32 amino acids from the C-terminal region of human General transcription and DNA repair factor IIH helicase subunit XPD (XPD/ERCC2).
      EpitopeC-terminus
      Clone22TF2-2F6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 22TF2-2F6 detects General transcription and DNA repair factor IIH helicase subunit XPD (ERCC2) in human cells. It targets an epitope with in 32 amino acids from the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ERCC2
      • XPD
      • XPDC
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight86.91 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected XPD in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1819-25UG 04054839558009