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07-2262 Anti-WNK3 Antibody

This WNK3 antibody is validated for use in WB for the detection of the WNK3 protein.

Anti-WNK3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: WNK3, PRKWNK3 UniProt Number: Q9BYP7
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07-2262
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2262
      DescriptionAnti-WNK3 Antibody
      Alternate Names
      • WNK lysine deficient protein kinase 3
      • serine/threonine-protein kinase WNK3
      • Protein kinase with no lysine 3
      • Protein kinase lysine-deficient 3
      • protein kinase, lysine deficient 3
      Background InformationWNK3 is a serine-threonine protein kinase and expressed throughout the nephron, but most commonly at intercellular junctions. In its active state, it is a promoter of NKCC2 and NCC activity. The opposite is true of WNK3 when in an inactive state. WNK3 changes the expression of these transporters in the membrane and is thought to be a regulator of blood pressure along with other members of the WNK family of protein kinases. In recent research, WNK3 has also been linked to regulating signaling cascades involved in tumorigenesis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis WNK3 antibody is validated for use in WB for the detection of the WNK3 protein.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human WNK3.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human and mouse.
      Other homologies: Rat (74% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
      Gene Symbol
      • WNK3
      • PRKWNK3
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryCATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. UniProtKB Q9H4A3

      COFACTOR: Magnesium (By similarity). UniProtKB Q9H4A3

      ENZYME REGULATION: Activation requires autophosphorylation of Ser-308. Phosphorylation of Ser-304 also promotes increased activity By similarity. UniProtKB Q9JIH7

      TISSUE SPECIFICITY: Expressed in brain, lung, kidney, liver and pancreas, and in fetal tissues including placenta, fetal brain, lung and kidney. Very low levels of expression were also detected in fetal heart, thymus, liver and spleen.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.

      Contains 1 protein kinase domain.

      CAUTION: Cys-176 is present instead of the conserved Lys which is expected to be an active site residue. Lys-159 appears to fulfill the required catalytic function. UniProtKB Q9JIH7
      Molecular Weight~220 kDa observed. Uncharacterized bands appear at ~52 and 60 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected WNK3 on 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-2262 04053252291807