Millipore Sigma Vibrant Logo

ABE1075 Anti-WD repeat-containing protein 5 Antibody

View Products on Sigmaaldrich.com
ABE1075
100 µL  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Fulfillment and Delivery Delayed
Fulfillment and Delivery Delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE1075
      DescriptionAnti-WD repeat-containing protein 5 Antibody
      Alternate Names
      • WD repeat-containing protein 5
      • BMP2-induced 3-kb gene protein
      Background InformationWD repeat-containing protein 5 (WDR5) or alternatively BMP2-induced 3kb gene protein, is encoded by the human gene named WDR5/BIG3 and is part of a family of WD-40 repeat proteins that appear to facilitate the formation of multi-protein complexes much like a sticky tape will bind a variety of components together. The WD repeat proteins are involved in a wide array of cellular functions and processes including cell cycle progression, homeobox gene activation, apoptosis, gene regulation and histone methylation. One of WDR5 best studied activities is as part of a multi-protein complex that facilitates the methylation of histones, particularly histone H3, through its interaction with MLL a histone methyltransferase. WDR5 is also expressed in developing bone cells including osteoblasts, chondrocytes, and marrow stromal cells and works to accelerate osteoblast differentiation and positively promotes Wnt signaling in developing bone tissues.
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-WD repeat-containing protein 5 antibody is validated for use in western blotting for the detection of WD repeat-containing protein 5.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot from an independent laboratory detected WD repeat containing protein 5 in tested cell lysates. (Wysocka, J., et al. (2003). Genes Dev. 17(7):896-911.; Tyagi, S., et al. (2007). 27(1):107-119.; Jin, S., et al. (2010). J Clin Invest. 120(2):593-606.; Tyagi, S., et al. (2009). EMBO J. 28(20):3185-3195.; Diao, Y., et al. (2012). Cell Stem Cell. 11(2):231-241.; Schram, A. W., et al. (2013). PLoS One. 8(7):e70035.).
      Biological Information
      ImmunogenLinear peptide corresponding to human WD repeat containing protein 5.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • WDR5
      • BIG3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~37 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysates.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WD repeat containing protein 5 in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1075 04053252956041