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MABN361 Anti-VAPA Antibody, clone 7E10.1

This Anti-VAPA Antibody, clone 7E10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of VAPA.

Anti-VAPA Antibody, clone 7E10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: VAPA, VAP33 UniProt Number: Q9P0L0
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MABN361
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN361
      DescriptionAnti-VAPA Antibody, clone 7E10.1
      Alternate Names
      • Vesicle-associated membrane protein-associated protein A
      • VAMP-A
      • VAMP-associated protein A
      • VAP-A
      • 33 kDa VAMP-associated protein
      • VAP-33
      • VAPA
      Background InformationVesicle-associated membrane protein-associated protein A (UniProt Q9P0L0; also known as 33 kDa VAMP-associated protein, hVAP-33, VAMP-A, VAMP-associated protein A, VAP-33, VAP-A) is encoded by the VAPA (also known as VAP33) gene (Gene ID 9218) in human. VAMP-associated proteins (VAPs) are type IV membrane proteins that are well conserved among species. There exist three human VAPs encoded by two genes, with VAPA encoding VAP-A and VAPB encoding VAP-B and VAP-C. VAPs generally localize at the endoplasmic reticulum (ER), although they are also reported to localize at other subcellular organelles in some species and cell types. VAP-A contains a major sperm protein (MSP) domain at the N-terminal, followed by a coiled-coil domain, and a transmembrane (TM) domain. VAPs were shown to have important roles in non-vesicular lipid transport, lipid metabolism, the regulation of ER structure, and the unfolded protein response through MSP domain-mediated interaction with FFAT motifs. Oxysterol-binding protein (OSBP) is a cytosolic receptor of cholesterol and oxysterols. OSBP is recruited to the ER by binding to the MSP domain of VAP-A, a process essential for the stimulation of sphingomyelin synthesis by 25-hydroxycholesterol.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-VAPA Antibody, clone 7E10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of VAPA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.2 µg/mL from a representative lot detected VAPA in 10 µg of mouse brain tissue lysate.
      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected VAPA in human cerebellum and human testis tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the C-terminal half of human VAPA.
      EpitopeC-terminal half
      Clone7E10.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityCross-reactivity to human VAPA spliced isoform 2 is highly expected, but not confirmed.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VAPA
      • VAP33
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~30 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected VAPA in 10 µg of SH-SY5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN361 04055977276992