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MABT1334-25UL Anti-Tropomyosin-4 Antibody, clone 2D2A12

Anti-Tropomyosin-4, clone 2D2A12, Cat. No. MABT1334, is a mouse monoclonal antibody that detects Tropomyosin delta-4 chain and is tested for use in Western Blotting.

MABT1334-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1334-25UL
      DescriptionAnti-Tropomyosin-4 Antibody, clone 2D2A12
      Alternate Names
      • Tropomyosin delta-4 chain
      • TM-4
      Background InformationTropomyosin alpha-4 chain (UniProt: P09495; also known as Tropomyosin-4, TM-4) is encoded by the Tpm4 gene (Gene ID: 24852) in rat. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Tropomyosin-4 1 is a cytoskeletal protein that associates with F-actin stress fibers and binds to actin filaments in muscle and non-muscle cells. It plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. It has a coiled coil structure formed by two polypeptide chains. It is abundantly expressed in kidney, lung, and spleen and is also observed in muscle and stomach tissues. Loss of Tpm4.1 is reported to lead to disturbance in cell-cell adhesions and invasive behavior in breast epithelial cells through Rac1-myosin IIB signaling. In colon cancer cells, TPM4 mRNA and protein expressions is shown to be significantly reduced and its low expression correlates with the clinal stage. Clone 2D2A12 detects both rat and human tropomyosin-4 and does not react with other exon1b containing isoforms from other tropomyosins. (Ref.: Schevzov, G., et al. (2011). BioArchitecture, 1 (4); 135-164; Jeong, S., et al. (2017). Oncotarget. 8(20); 33544-33559; Yang, R., et al. (2018). Biomed. Pharmacother. 101; 1-7).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Tropomyosin-4, clone 2D2A12, Cat. No. MABT1334, is a mouse monoclonal antibody that detects Tropomyosin delta-4 chain and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Tropomyosin-4 in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of rat Tropomysin-4 corresponding to exon 1b in Tpm4 gene.
      Clone2D2A12
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2D2A12 is a mouse monoclonal antibody that detects rat Tropomyosin-4. It targets an epitope within 19 amino acids from the N-terminal region corresponding to exon 1b in Tpm4 gene.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tpm4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~30 kDa observed; 28.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from mouse skeletal muscle (Negative control) and human BJ fibroblasts (Positive control).

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Tropomyosin-4 in lysate from human BJ fibroblasts, but not in mouse skeletal muscle extract (Negative control).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1334-25UL 04061841844426

      Documentation

      Anti-Tropomyosin-4 Antibody, clone 2D2A12 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tropomyosin-4 Antibody, clone 2D2A12 Certificates of Analysis

      TitleLot Number
      Anti-Tropomyosin-4, clone 2D2A12 - Q3438675 Q3438675

      References

      Reference overviewPub Med ID
      Tropomyosin isoforms and reagents
      Galina Schevzov 1 , Shane P Whittaker, Thomas Fath, Jim Jc Lin, Peter W Gunning
      Bioarchitecture  1(4)  135-164  2011

      Show Abstract       		22069507 22069507