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AB10406 Anti-TRAM1 Antibody

Detect TRAM1 using this Anti-TRAM1 Antibody validated for use in WB, IH(P).

Anti-TRAM1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: PRO1292, TRAM1, TRAMP1, PNAS8 UniProt Number: Q15629
AB10406
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB10406
      DescriptionAnti-TRAM1 Antibody
      Alternate Names
      • translocation associated membrane protein 1
      • translocation-associating membrane protein 1
      • translocating chain-associating membrane protein
      Background InformationTranslocating chain-associated membrane protein 1 (TRAM1) belonging to the TRAM family is a multi-pass membrane protein, a component of the translocon, found within the Endoplasmic Reticulum (ER). TRAM1 functions as a mediator for the lateral movement of specific signaling peptides and transmembrane segments into the membrane bilayer from the translocon. However, TRAM1 is not necessary for all transmembrane proteins to translocate into the ER. Studies indicate a possible role for TRAM1 in dislocating membrane glycoprotein, which would enable TRAM1 to have a dual role in nascent polypeptide processing and ER protein dislocation.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human liver lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationDetect TRAM1 using this Anti-TRAM1 Antibody validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blot (SNAP ID) Analysis: 1 µg/mL of this antibody has been shown to detect TRAM1 in 10 µg of human liver lysate.

      Immunohistochemistry Analysis: A 1:500 dilution of this antibody has been shown to detect TRAM1 in squamous epithelial carcinoma tissue, with staining patterns restricted to malignant cells.
      Biological Information
      ImmunogenLinear peptide corresponding to the internal domain of human TRAM1.
      EpitopeInternal Domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes TRAM1.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. [provided by RefSeq].
      Gene Symbol
      • PRO1292
      • TRAM1
      • TRAMP1
      • PNAS8
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Stimulatory or required for the translocation of secretory proteins across the ER membrane.

      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.

      SEQUENCE SIMILARITIES: Belongs to the TRAM family.

      Contains 1 TLC (TRAM/LAG1/CLN8) domain.
      Molecular Weight~36 kDa observed (43 kDa calculated molecular weight).
      Western blot analysis shows that TRAM1 is an abundantly expressed, approximately 36 kDa microsomal glycoprotein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins (Oresic, K., et al. JBC (2009). 284:5905-5914.).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human liver lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected TRAM1 in 10 µg of human liver lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB10406 04053252628832