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ABE434 Anti-TIF1-alpha Antibody

Detect TIF1α using this rabbit polyclonal antibody, Anti-TIF1-alpha Antibody validated for use in western blotting & ICC.

Anti-TIF1-alpha Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: TRIM24, RNF82, TIF1, TIF1A UniProt Number: O15164
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ABE434
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE434
      DescriptionAnti-TIF1-alpha Antibody
      Alternate Names
      • Transcription intermediary factor 1-alpha
      • E3 ubiquitin-protein ligase TRIM24
      • RING finger protein 82
      • Tripartite motif-containing protein 24
      Background InformationTranscription intermediary factor 1-alpha (TIF1-alpha) is also called E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, and Tripartite motif-containing protein 24. TIF1-alpha is a transcriptional coactivator that interacts with chromatin depending on histone H3 modifications, with the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). TIF1-alpha has E3 protein-ubiquitin ligase activity, helps regulate cell proliferation and apoptosis and up-regulates transcription activation. Aberrant TIF1-alpha can cause thyroid papillary carcinoma (TPC) and is up-regulated in some breast cancers.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect TIF1α using this rabbit polyclonal antibody, Anti-TIF1-alpha Antibody validated for use in western blotting & ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a represenative lot detected TIF1-alpha in HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of the Nuclear Receptor Binding Site (NRBS) of human TIF1-alpha.
      EpitopeC-terminus of the Nuclear Receptor Binding Site (NRBS)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the C-terminus of the Nuclear Receptor Binding Site (NRBS) of TIF1-alpha.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human and Mouse. Predicted to react with Rhesus Macaque based on 100% sequence homology. Other homologies: Rat (93% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TRIM24
      • RNF82
      • TIF1
      • TIF1A
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~140 kDa observed. Uniprot descrives 2 isoforms at ~117 kDa and ~113 kDa due to alternative splicing. This protein has been observed at ~140 kDa (Gunawardena, H., et al. (2008). Rheumatology (Oxford). 47(3):324-328.).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected TIF1-alpha in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE434 04053252914676