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MABE1079 Anti-TAF10/TAFII30 Antibody, clone 6TA-2B11

Anti-TAF10Antibody, clone 6TA-2B11 is an antibody against TAFII30 for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.

Anti-TAF10/TAFII30 Antibody, clone 6TA-2B11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: TAF10, TAF2A, TAF2H, TAFII30 UniProt Number: Q12962
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MABE1079
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IPMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABE1079
      DescriptionAnti-TAF10/TAFII30 Antibody, clone 6TA-2B11
      Alternate Names
      • Transcription initiation factor TFIID subunit 10
      • STAF28
      • TAF(II)30
      • TAFII-30
      • TAFII30
      • Transcription initiation factor TFIID 30 kDa subunit
      Background InformationTranscription initiation factor TFIID subunit 10 (UniProt Q12962; also known as STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit) is encoded by the TAF10 (also known as TAF2A, TAF2H, TAFII30) gene (Gene ID 6881) in human.Transcription initiation factor TFIID subunit 10 (UniProt Q12962; also known as STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit) is encoded by the TAF10 (also known as TAF2A, TAF2H, TAFII30) gene (Gene ID 6881) in human. TAF10 (TAFII30) is a subunit of both the general transcription factor (GTF) TFIID and the coactivator SAGA (Spt-Ada-Gcn5-acetyltransferase) histone acetyltransferase (HAT) complexes involved in the initiation of RNA polymerase II (Pol II) transcription in eukaryotes. TFIID complex is composed of the TATA-binding protein (TBP) and thirteen TBP-associated factors (TAFs) that allow its recognition of not only TATA box-containing, but also TATA-less promoters for site-specific assembly of preinitiation complex (PIC). TAF10-knockout studies in mice indicate that TAF10 plays an important role during embryonic development and homeostasis in a tissue-dependent manner. TAF10 ablation is reported to result in severe anemia and lethality at embryonic stage E13.5 due to impaired erythropoiesis. In addition, TAF10 is shown to not only interact with the transcription factor GATA1, but also regulate its transcription as evidenced by its occupancy at GATA1 genomic locus in human fetal erythroid cells.
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1κ ascites with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-TAF10Antibody, clone 6TA-2B11 is an antibody against TAFII30 for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A 1:200 dilution from a representative lot detected TAF10/TAFII30 in A431 and HeLa cells.
      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TAF10/TAFII30 in TAFIID complex isolated from HeLa nuclear extract (Courtesy of Dr. Mustapha OULAD-ABDELGHANI, IGBMC, France).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated TAF10 together with associated GATA1 from murine erythroleukemia (MEL) cell nuclear extracts (Papadopoulos, P., et al. (2015). Mol. Cell. Biol. 35(12):2103-2118).
      Western Blotting Analysis: A representative lot detected TAF10 in E12.5 mouse fetal liver nuclear extracts and murine erythroleukemia (MEL) cell nuclear extracts, as well as in GATA1 immunoprecipitate from MEL cell and fetal liver nuclear extracts (Papadopoulos, P., et al. (2015). Mol. Cell. Biol. 35(12):2103-2118).
      Immunocytochemistry Analysis: A representative lot detected TAF10 immunoreactivity in trophoblasts from 3-day cultured wild-type, but not Taf10-/-mouse embryonic blastocysts isolated at 3.5 dpc (days post-coitum) by immunofluorescent staining of 1% paraformaldehyde-fixed, 0.1% Tween 20-permeabilized blastocysts (Mohan, W.S. Jr, et al. (2003). Mol .Cell. Biol. 23(12):4307-4318).
      Biological Information
      ImmunogenRecombinant human TAF10/TAFII30.
      EpitopeInternal (N-terminal half).
      Clone6TA-2B11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6TA-2B11 (a.k.a. 6TA 2B11) specifically immunostained wild-type, but not Taf10-/-, mouse embryonic blastocysts (Mohan, W.S. Jr, et al. (2003). Mol .Cell. Biol. 23(12):4307-4318).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TAF10
      • TAF2A
      • TAF2H
      • TAFII30
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~30 kDa observed. 21.71 kDa calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected TAF10/TAFII30 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1079 04055977344165