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MABC586 Anti-Sulf-2 Antibody, clone 8G1

This Anti-Sulf-2 Antibody, clone 8G1 is validated for use in Western Blotting and Immunocytochemistry for the detection of Sulf-2.

Anti-Sulf-2 Antibody, clone 8G1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Tumor Markers Gene Symbol: SULF2, KIAA1247 UniProt Number: Q8IWU5
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MABC586
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC586
      DescriptionAnti-Sulf-2 Antibody, clone 8G1
      Alternate Names
      • Extracellular sulfatase Sulf-2
      • hSulf-2
      Background InformationSulf-2 is an endoplasmic reticulum enzyme that is involved in sulfur metabolism which is important in bone, cartilage, and skeletal development. Sulf-2 is also important in neurological situations including myelination and glial cell differentiation and GDNF activity. Sulf-2 activity is also found in the kidney and important in glomerular basement membrane development and glomerular function. Finally, Sulf-2 is involved in heparan sulfate metabolism. Sulf-2 removes sulfate moieties on heparan sulfate proteoglycans which in turn modulate the heparin binding growth factor signaling activity of the cell. Sulf-2 also impacts extracellular matrix interactions and it is important during development and growth, as well as Wnt signaling and VEGF production by endothelial cells during vascularization. In disease, Sulf-2 expression in cancer is upregulated and inhibition of Sulf-2 limits cancer progression and showed increased apoptosis within tumors, thus Sulf-2 activation is being examined as a potential anti-cancer therapy.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Sulf-2 Antibody, clone 8G1 is validated for use in Western Blotting and Immunocytochemistry for the detection of Sulf-2.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: 4 µg/mL of a representative lot of this antibody detected Sulf-2 in Sulf-2 transfected HEK293 cell lysates.

      Immunocytochemistry Analysis: 4 µg/mL of a representative lot of this antibody detected Sulf-2 in Sulf-2 transfected HEK293 cells, but not in HEK293 cells transfected with an empty vector or Sulf-1.
      Biological Information
      ImmunogenRecombinant protein corresponding to Human Sulf-2.
      Clone8G1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SULF2
      • KIAA1247
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~125 & 75 kDa observed.
      Sulf-2 is synthesized as a ~125 kDa pre-protein and is subsequently cleaved into ~75 kDa and ~50 kDa fragments, which are held together by disulfide bonds in the mature protein.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 conditioned media.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sulf-2 in 10 µg of MCF-7 conditioned media.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC586 04055977109313

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies