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MABC1037 Anti-Sp100 Antibody, clone 2H1.1

Anti-Sp100 Antibody, clone 2H1.1 is an antibody against Sp100 for use in Western Blotting, Immunocytochemistry.

Anti-Sp100 Antibody, clone 2H1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Chromatin Biology Gene Symbol: SP100 UniProt Number: P23497
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MABC1037
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1037
      DescriptionAnti-Sp100 Antibody, clone 2H1.1
      Alternate Names
      • Nuclear autoantigen Sp-100
      • Nuclear dot-associated Sp100 protein
      • Speckled 100 kDa
      Background InformationNuclear autoantigen Sp-100 (UniProt P23497; also known as Nuclear dot-associated Sp100 protein, Speckled 100 kDa) is encoded by the SP100 gene (Gene ID 6672) in human. The SP100 family genes, including SP100, SP110, SP140 and SP140L, encode highly similar proteins mainly expressed in leukocytes. Interferons (IFNs) and other viral infection-related stimuli induce SP100 family gene expression and mutations of SP100 genes are linked to immune diseases and cancers. SP100 colocalizes with promyelocytic leukemia (PML) protein in nuclear bodies (NBs), also known as nuclear domain 10 (ND10), that function to regulate gene transcription via chromatin-based mechanisms and thereby mediate a variety of cellular functions including senescence, apoptosis, protein degradation, telemere stasbility, and defense against viral infections.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Sp100 Antibody, clone 2H1.1 is an antibody against Sp100 for use in Western Blotting, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Sp100 nuclear foci in HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant human Sp100 internal fragment.
      EpitopeInternal (N-terminal half).
      Clone2H1.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2H1.1 targeted epitope region is present in all seven human Sp100 spliced isoforms reported by UniProt (P23497).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SP100
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~100/78 kDa observed. 100.3 kDa (isoform Sp100-HMG/SP100HMG/SpAlt-HMG), 53.64 kDa (isoform Sp100-A/SP100A/SP100), 78.43 kDa (isoform Sp100-B/SP100B/SpAlt-212), 101.4 kDa (isoform Sp100-C/SP100C), 52.98 kDa (isoform SpAlt-C), 50.46 kDa (isoform 6), and 49.90 kDa (isoform 7) calculated (with Met1 removed). Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 nuclear extract.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected Sp100 in 10 µg of HepG2 nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1037 04055977350890