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ABE2868-25UL Anti-Sox-9

Anti-Sox-9, Cat. No. ABE2868-25UL, is a highly specific rabbit polyclonal antibody, that targets Transcription factor SOX-9 and has been tested in Immunofluorescence, Immunocytochemistry, Western Blotting and Immunohistochemistry (Paraffin).

Anti-Sox-9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SOX9 UniProt Number: P48436
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ABE2868-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MIF, ICC, WB, IH(P)RbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2868-25UL
      DescriptionAnti-Sox-9
      Alternate Names
      • Transcription factor SOX-9
      Background InformationTranscription factor SOX-9 (UniProt P48436; also known SOX-9) is encoded by the SOX9 gene (Gene ID 6662) in human. SOX family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences encoding for the HMG-box domains that mediate sequence-specific DNA binding. SOX genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of SOX genes cluster at about 40 genomic loci and at least 30 SOX genes have been identified. SOX-9 contains one HMG box DNA-binding domain and acts as a key regulator of developmental processes, including male sex determination, normal skeletal development, chondrogenesis, neurogenesis, and neural crest development. SOX-9 is acts downstream of sonic hedgehog signaling and is shown to be essential for the maintenance of multipotent neural stem cells in embryonic and adult central nervous system (CNS). It is not detected in neuroblasts of the adult subependymal zone (SEZ); however, it is expressed by transit amplifying cells and ependymal cells in the same region. SOX-9 is also shown to be essential for the 'switch' from neurogenesis to gliogenesis in caudal regions of the developing CNS. SOX-9 up-regulation is reported in cancers of various origins, including breast, colon, prostate, and lung, where its expression correlates with malignancy and patient survival. (Ref.: Scott, CE et al. (2010). Nat. Neurosci. 13(10): 1181-89).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antiserum with 0.02% sodium azide.
      Applications
      ApplicationAnti-Sox-9, Cat. No. ABE2868-25UL, is a highly specific rabbit polyclonal antibody, that targets Transcription factor SOX-9 and has been tested in Immunofluorescence, Immunocytochemistry, Western Blotting and Immunohistochemistry (Paraffin).
      Key Applications
      • Immunofluorescence
      • Immunocytochemistry
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected SOX-9 in 10 µg of HepG2 cell lysate.
      Western Blotting Analysis: A representative lot detected SOX-9 in Western Blotting applications (Augereau, C., et. al. (2016). Hum Mol Genet. Epub ahead of print).
      Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected SOX-9 in HEK293T cells transfected with Sox-9 ( Courtesy of Dr. Patrick Jacquemin at Université catholique de Louvain ).
      Immunofluorescence Analysis: A representative lot detected SOX-9 in PFA-fixed, paraffin-embedded pancreatic sections from an adult mouse; ductus (Augereau, C., et. al. (2016). Hum Mol Genet. 25(22):5017-5026).
      Immunohistochemistry Analysis: A representative lot detected SOX-9 in PFA-fixed, paraffin-embedded liver (left) and pancreatic sections (right) of E14.5 mouse embryo, PFA-fixed, paraffin-embedded intestine sections of E14.5 mouse embryo (Augereau, C., et. al. (2016). Hum Mol Genet. 25(22):5017-5026).
      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SOX-9 in e14.5 mouse liver and e14.5 mouse pancreas tissues.
      Immunocytochemistry Analysis: A representative lot detected SOX-9 in PFA-fixed 3T3 cells overexpressing Sox9 (Augereau, C., et. al. (2016). Hum Mol Genet. 25(22):5017-5026).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal end of human Sox-9.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit ployclonal antibody detects SOX-9 in human, mouse, and rat cells. It targets an epitope with in 24 amino acids from the C-terminal end.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Human, Rat.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • SOX9
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight65 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in L6 cell lysate.
      Western Blotting Analysis: A 1:500 dilution of this antibody detected SOX-9 in 10 µg of L6 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2868-25UL 04054839474927