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05-1459 Anti-SMAD1 Antibody, clone AS22

Anti-SMAD1 Antibody, clone AS22 detects level of SMAD1 & has been published & validated for use in WB & IF.

Anti-SMAD1 Antibody, clone AS22 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: BSP-1 , BSP1, CMT2D , GARS, HMN5, JV4-1, JV41, MADH1, MADR1 , hSMAD1 UniProt Number: Q15797
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05-1459
200 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1459
      Replaces04-1100
      DescriptionAnti-SMAD1 Antibody, clone AS22
      Alternate Names
      • SMAD 1
      • SMAD family member 1
      • SMAD
      • mothers against DPP homolog 1
      • MAD
      • mothers against decapentaplegic homolog 1
      • Mad-related protein 1
      • Mothers against DPP homolog 1
      • Sma- and Mad-related protein 1
      • TGF-beta signaling protein 1
      • Transforming growth factor-beta-signaling protein 1
      Background InformationSmad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SMAD1 Antibody, clone AS22 detects level of SMAD1 & has been published & validated for use in WB & IF.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesWestern Blot Analysis:
      This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.
      Biological Information
      ImmunogenGST-fusion protein corresponding to amino acids 1-110 of human SMAD1.
      CloneAS22
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityDetects SMAD1
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and Mouse. Other species were not tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]
      Gene Symbol
      • BSP-1
      • BSP1
      • CMT2D
      • GARS
      • HMN5
      • JV4-1
      • JV41
      • MADH1
      • MADR1
      • hSMAD1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).
      SUBUNIT: Interacts with HGS, NANOG and ZCCHC12 (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
      TISSUE SPECIFICITY: Ubiquitous. Highest expression seen in the heart and skeletal muscle.
      PTM: Phosphorylated on serine by BMP type 1 receptor kinase.
      PTM: Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
      Molecular Weight52 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot

      Western Blot Analysis:
      This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-1459 04053252282690

      Documentation

      Anti-SMAD1 Antibody, clone AS22 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SMAD1 Antibody, clone AS22 Certificates of Analysis

      TitleLot Number
      Anti-SMAD1, clone AS22 - 2007686 2007686
      Anti-SMAD1, clone AS22 - 2279259 2279259
      Anti-SMAD1, clone AS22 - 3259069 3259069
      Anti-SMAD1, clone AS22 - 4108259 4108259
      Anti-SMAD1, clone AS22 - DAM1605006 DAM1605006
      Anti-SMAD1, clone AS22 - DAM1616394 DAM1616394
      Anti-SMAD1, clone AS22 - NG1548165 NG1548165
      Anti-SMAD1, clone AS22 - NG1862037 NG1862037
      Anti-SMAD1, clone AS22 -2632491 2632491
      Anti-SMAD1, clone AS22 -2700786 2700786
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies