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MABF2213-25UG Anti-SARS-CoV ORF7a Antibody, clone 3C9

Anti-SARS-CoV ORF7a, clone 3C9, Cat. No. MABF2213, is a mouse monoclonal antibody that detects of SARS-CoV ORF7a protein and is tested for use in Immunofluorescence and Western Blotting.

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MABF2213-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2213-25UG
      DescriptionAnti-SARS-CoV ORF7a Antibody, clone 3C9
      Alternate Names
      • ORF7a protein
      • Accessory protein 7a
      • Protein U122
      • Protein X4
      Background InformationORF7a protein (UniProt: P59635; also known as Accessory protein 7a, Protein U122, Protein X4) is encoded by the 7a gene (Gene ID: 1489674) in Severe acute respiratory syndrome coronavirus (SARS-CoV). ORF7a protein is expressed by the severe acute respiratory syndrome coronavirus (SARS-CoV) and is not found in other known coronaviruses. It is synthesized with a signal peptide (aa 1-15), which is subsequently cleaved off. Its presence is detected in the mitochondria and the endoplasmic reticulum-Golgi intermediate compartments. The C-terminal region of ORF7a contains a transmembrane domain (aa 97-117) and a short cytoplasmic tail (118 to 122 aa). The transmembrane domain is shown to be important for the insertion of ORF7a into intracellular membranes. The cytoplasmic tail has an endoplasmic reticulum retrieval motif that mediates the transport of ORF7a between the Golgi and the endoplasmic reticulum. ORF7 can induce apoptosis in various cell lines, however, overexpression of Bcl-XL can block ORF-7a induced apoptosis. It is not shown to react with any pro-apoptotic proteins of theBcl-2 family. Its transmembrane domain is also important for its interaction with Bcl-XL and for the induction of apoptosis. Two residues (aa 224, 225) within the transmembrane domain of Bcl-XL are essential for its interaction with ORF7a. SARS-CoV-2 ORF7a is considered as an immunomodulating factor for immune cell binding and can trigger inflammatory responses. It can interact with CD14+ monocytes with high efficiency, compared to the SARS-CoV ORF7a. (Ref.: Zhou, Z., et al. (2021). iScience 24(3); 102187; Tan, YX., et al. (2007). J. Virol. 81(12); 6346-6355).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-SARS-CoV ORF7a, clone 3C9, Cat. No. MABF2213, is a mouse monoclonal antibody that detects of SARS-CoV ORF7a protein and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected SARS-CoV ORF7a in Western Blotting applications (Tan, Y-X., et al. (2007). J Virol. 81(12):6346-55).

      Immunofluorescence Analysis: A representative lot detected SARS-CoV ORF7a in Immunofluorescence applications (Tan, Y-X., et al. (2007). J Virol. 81(12):6346-55).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant, full-length ORF7a protein of SARS-CoV (without the signal peptide).
      EpitopeUnknown
      Clone3C9
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3C9 is a mouse monoclonal antibody that detects ORF7a protein of SARS-CoV.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteSARS-CoV
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • 7a
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~14 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant SARS-CoV-2 ORF7a protein.

      Western Blotting Analysis: 1:100,000 dilution of this antibody detected recombinant SARS-CoV-2 ORF7a protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2213-25UG 04065267327785