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CB1040 Anti-S100B Mouse mAb (1B2)

This Anti-S100B Mouse mAb (1B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of S100B.

Anti-S100B Mouse mAb (1B2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-S100 Calcium Binding Protein B

CB1040
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~10 kDa S100B protein in HepG2 cells and human salivary gland tissue.
      Catalogue NumberCB1040
      Brand Family Calbiochem®
      SynonymsAnti-S100 Calcium Binding Protein B
      Application Data
      Detection of human S100B by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-S100B Mouse mAb (1B2) (Cat. No. CB1040) (1-5 µg/ml). Detection: chemiluminescences.

      Detection of human S100B by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-S100B Mouse mAb (1B2) (Cat. No. CB1040) (3 µg/ml). Detection: colorimentric (DAB)
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHepG2 cells, Human salivary gland tissue
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 1-93 human S100B, expressed as a GST fusion protein
      Clone1B2
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CB1040 0

      Documentation

      Anti-S100B Mouse mAb (1B2) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-S100 Calcium Binding Protein B
      ApplicationImmunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human S100B by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-S100B Mouse mAb (1B2) (Cat. No. CB1040) (1-5 µg/ml). Detection: chemiluminescences.

      Detection of human S100B by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-S100B Mouse mAb (1B2) (Cat. No. CB1040) (3 µg/ml). Detection: colorimentric (DAB)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~12 kDa S100B protein.
      BackgroundThe protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 1-93 human S100B, expressed as a GST fusion protein
      Clone1B2
      IsotypeIgG₁
      Specieshuman
      Positive controlHepG2 cells, Human salivary gland tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review