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MABF3184-100UG Anti-RANTES/CCL5 Antibody, clone 15B5.6

Anti-RANTES/CCL5, clone 15B5.6, Cat. No. MABF3184, is a mouse monoclonal antibody that detects RANTES/CCL5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABF3184-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3184-100UG
      DescriptionAnti-RANTES/CCL5 Antibody, clone 15B5.6
      Alternate Names
      • C-C motif chemokine 5
      • EoCP
      • Eosinophil chemotactic cytokine
      • SIS-delta
      • Small-inducible cytokine A5
      • T cell-specific protein P228
      • TCP228
      • T-cell-specific protein RANTES
      Background InformationC-C motif chemokine 5 (UniProt: P13501; also known as EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, TCP228, T-cell-specific protein RANTES, CCL5) is encoded by the CCL5 (also known as D17S136E, SCYA5) gene (Gene ID: 6352) in human. CCL5 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature protein (aa 24-91), which can further into RANTES (3-68) and RANTES (4-68). CCL5 serves as a chemoattractant for monocytes, memory T-helper cells, and eosinophils. It promotes immune cell recruitment to sites of inflammation. It also causes the release of histamine from basophils. It is involved in the activation of several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. The CCL5 activation of pancreatic cancer cells expressing CCR5 is reported to increase their invasive potential. CCL5 also functions as one of the natural ligands for the CCR5 and is reported to suppress in vitro replication of the R5 strains of HIV-1 that uses CCR5 as a coreceptor. The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a shown to be a potent inhibitor of HIV-1-infection. RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(3-68). (Ref.: Singh, SK., et al. (2018). Sci. Rep. 8; Article 1323; Lim, JK., et al. (2005). J. Leukoc Biol. 78(2); 442-452).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-RANTES/CCL5, clone 15B5.6, Cat. No. MABF3184, is a mouse monoclonal antibody that detects RANTES/CCL5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected RANTES/CCL5 in human spleen tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length recombinant mature human RANTES/CCL5 (without the signal peptide).
      EpitopeUnknown
      Clone15B5.6
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 15B5.6 is a mouse monoclonal antibody that detects RANTES/CCL5.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CCL5
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~8 kDa observed; 9.99 kDa calculated for the whole molecule. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human RANTES/CCL5.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human RANTES/CCL5.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3184-100UG 04065267614007