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MABS1758 Anti-RAD9A Antibody, clone 5H8.1

Detect RAD9A using this mouse monoclonal Anti-RAD9A Antibody, clone 5H8.1, Cat. No. MABS1758, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-RAD9A Antibody, clone 5H8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: RAD9A, RAD9 UniProt Number: Q99638
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MABS1758
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1758
      DescriptionAnti-RAD9A Antibody, clone 5H8.1
      Alternate Names
      • Cell cycle checkpoint control protein RAD9A
      • DNA repair exonuclease rad9 homolog A
      • hRAD9
      • Rad9-like protein
      Background InformationCell cycle checkpoint control protein RAD9A (EC 3.1.11.2; UniProt Q99638; also known as DNA repair exonuclease rad9 homolog A, hRAD9, Rad9-like protein) is encoded by the RAD9A (also known as RAD9A) gene (Gene ID 5883) in human. RAD9A is a component of the trimeric PCNA-like 9-1-1 complex (Rad9A, Rad1, and Hus1) and plays an important role in maintaining genomic integrity. RAD9A participates in both ATR and ATM signaling pathways-mediated cell cycle checkpoints in mitosis. Evidences indicate that RAD9A participates in DNA damage repair by interacting with proteins involved in base excision repair, mismatch repair and homologous recombination repair. RAD9A is shown to colocalize with γH2AX following DNA damage induction in an ATM-independent manner, and RAD9A inactivation delays the appearance of ionizing radiation-induced γH2AX foci. Rad9a-knockout causes embryonic lethality in mice due to increased apoptotic activity and reduced cellular proliferation, while mice heterozygous for Rad9a are prone to spontaneous and radiation-induced cataractogenesis. In addition, conditional knockout in keratinocytes causes enhanced susceptibility to carcinogen-induced skin tumors, while conditional Rad9a deletion in undifferentiated spermatogonia results in drastic effects on meiosis.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect RAD9A using this mouse monoclonal Anti-RAD9A Antibody, clone 5H8.1, Cat. No. MABS1758, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected nuclear RAD9A immunoreactivity in human prostate cancer, pancreas, and small intestine tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human RAD9A ABL1-interacting domain fragment.
      Clone5H8.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5H8.1 targeted epitope lies within RAD9A ABL1-interacting domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAD9A
      • RAD9
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~42 kDa observed. 42.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human skeletal muscle tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected RAD9A in 10 µg of human skeletal muscle tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1758 04054839114526