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539229 Anti-Protein Disulfide Isomerase Rabbit pAb

This Anti-Protein Disulfide Isomerase Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, IP, Paraffin Sections for the detection of Protein Disulfide Isomerase.

Anti-Protein Disulfide Isomerase Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-PDI

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539229
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, Gp, Ht, H, Mk, M, Porcine, R, Sh, XnRbPolyclonal Antibody

Pricing & Availability

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539229-50UL
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      Description
      OverviewRecognizes the ~55 kDa (apparent MW) protein disulfide isomerase protein.
      Catalogue Number539229
      Brand Family Calbiochem®
      SynonymsAnti-PDI
      Application Data
      Detection of protein disulfide isomerase immunoblotting. Samples: Purified bovine PDI (lane 1) and whole cell lysate from HeLa cells (lane 2). Primary antibody: Anti-Protein Disulfide Isomerase Rabbit pAb (Cat. No. 539229) (1:1000). Detection: chemiluminescence.
      References
      ReferencesCribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther. 282, 1064.
      Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Positive controlHeLa cells, rat testis, mouse spleen
      Preservative≥0.9% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (1:1000, colorimetric)
      Immunocytochemistry (see comments)
      Immunoprecipitation (see comments)
      Paraffin Sections (see comments)
      Application CommentsThis antibody will also work in immunocytochemistry, immunoprecipitation, and paraffin sections; the concentration must be determined for each sample analyzed. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogenbovine liver Protein Disulfide Isomerase
      ImmunogenBovine
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Canine
      • Guinea Pig
      • Hamster
      • Human
      • Monkey
      • Mouse
      • Porcine
      • Rat
      • Sheep
      • Xenopus
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      539229-50UL 04055977268973

      Documentation

      Anti-Protein Disulfide Isomerase Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Protein Disulfide Isomerase Rabbit pAb Certificates of Analysis

      TitleLot Number
      539229

      References

      Reference overview
      Cribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther. 282, 1064.
      Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-March-2012 JSW
      SynonymsAnti-PDI
      ApplicationImmunoblotting (1:1000, colorimetric)
      Immunocytochemistry (see comments)
      Immunoprecipitation (see comments)
      Paraffin Sections (see comments)
      Application Data
      Detection of protein disulfide isomerase immunoblotting. Samples: Purified bovine PDI (lane 1) and whole cell lysate from HeLa cells (lane 2). Primary antibody: Anti-Protein Disulfide Isomerase Rabbit pAb (Cat. No. 539229) (1:1000). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~55 kDa (apparent MW) protein disulfide isomerase protein.
      BackgroundPDI is an abundant component of the ER that catalyzes disulfide bond formation in the biosynthesis of various secretory and cell surface proteins. It is a multi-functional polypeptide identical to the β-subunit of prolyl 4-hydroxylase and a cellular thyroid hormone-binding protein. It has two thioredoxin-like regions, each con-taining a Cys-Gly-His-Cys sequence that may represent two independently acting catalytic sites of PDI activity. The C-terminus of PDI has a KDEL sequence, which acts as retention signal for proteins of the ER lumen. PDI exists as a homodimer of ~107 kDa. Prolyl-4-hydroxylase, an α2β2 tetramer, catalyzes the formation of 4-hydroxyproline in collagen by the hydroxylation of proline residues in peptide linkages. The PDI/β-subunit of prolyl-4-hydroxylase is required for its retention in the ER.
      HostRabbit
      Immunogen speciesBovine
      Immunogenbovine liver Protein Disulfide Isomerase
      IsotypeIgG
      SpeciesXenopus, bovine, canine, guinea pig, hamster, human, monkey, mouse, porcine, rat, sheep
      Positive controlHeLa cells, rat testis, mouse spleen
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Preservative≥0.9% sodium azide
      CommentsThis antibody will also work in immunocytochemistry, immunoprecipitation, and paraffin sections; the concentration must be determined for each sample analyzed. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther. 282, 1064.
      Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.