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MABC612 Anti-Procadherin FAT1 Antibody, clone C257

This Anti-Procadherin FAT1 Antibody, clone C257 is validated for use in Western Blotting and Immunoprecipitation for the detection of Procadherin FAT1.

Anti-Procadherin FAT1 Antibody, clone C257 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: CDHF7, FAT UniProt Number: Q14517
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MABC612
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC612
      DescriptionAnti-Procadherin FAT1 Antibody, clone C257
      Alternate Names
      • Cadherin family member 7
      • Cadherin-related tumor suppressor homolog
      • Protein fat homolog
      Background InformationProcadherin FAT1 protein is a cell membrane bound protein that also has an active nuclear-translocation component that can alter specific gene expression and cell proliferation. Procadherin FAT1 is important in cell polarization and directed cell migration and also modulates cell-cell contact interactions. Procadherin FAT1 is expressed in epithelial, endothelial and smooth muscle cells and plays roles in their response to injury and is often highly induced after arterial injury and effects vascular smooth muscle cell growth and migration. Procadherin FAT1 is also important in cancer where it appears to function as both an oncogene and a tumor suppressor depending upon the cell context. Also recent research has shown that soluble FAT1 can be a novel biomarker candidate for such cancers as pancreatic cancer.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Procadherin FAT1 Antibody, clone C257 is validated for use in Western Blotting and Immunoprecipitation for the detection of Procadherin FAT1.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected Procadherin FAT1 in 10 µg of FAT1 transfected MV3 cell lysate.
      Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Procadherin FAT1 in 10 µg of FAT1 transfected MDA-MB-231 and MDA-MB-468 cell lysate.
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Procadherin FAT1 in C257 cell lysate. (Courtesy of Dr. R. Thorne, U of Newcastle, AU.)
      Biological Information
      ImmunogenRecombinant protein corresponding to Human FAT1.
      CloneC257
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDHF7
      • FAT
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight>260 kDa observed. Procadherin FAT1 is an extremely large cell surface receptor with a calculated MW of 506 kDa and it's highly glycosolated. It may be difficult to resolve the protein by western blot. An uncharacterized band at 50 kDa and other lower MWs may be observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in FAT1 transfected NIH-3T3 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Procadherin FAT1 in 10 µg of FAT1 transfected NIH-3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC612 04055977112191

      Documentation

      Anti-Procadherin FAT1 Antibody, clone C257 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Procadherin FAT1 Antibody, clone C257 Certificates of Analysis

      TitleLot Number
      Anti-Procadherin FAT1, clone C257 Q2473150
      Anti-Procadherin FAT1, clone C257 - 3176724 3176724
      Anti-Procadherin FAT1, clone C257 - 3871238 3871238
      Anti-Procadherin FAT1, clone C257 - 3996828 3996828
      Anti-Procadherin FAT1, clone C257 - 4146502 4146502
      Anti-Procadherin FAT1, clone C257 - 4187066 4187066
      Anti-Procadherin FAT1, clone C257 Monoclonal Antibody 2945576
      Anti-Procadherin FAT1, clone C257 Monoclonal Antibody 2960439
      Anti-Procadherin FAT1, clone C257 Monoclonal Antibody 3012760
      Anti-Procadherin FAT1, clone C257 Monoclonal Antibody 3107341