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MABN1186 Anti-Prion Protein Antibody, clone 12D6.1

This Anti-Prion Protein Antibody, clone 12D6.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Prion Protein.

Anti-Prion Protein Antibody, clone 12D6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: PRNP, ALTPRP, CJD, GSD, HDL1, KURU, PRIP, PRP, SENF UniProt Number: P04156
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MABN1186
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1186
      ReplacesMAB1562
      DescriptionAnti-Prion Protein Antibody, clone 12D6.1
      Alternate Names
      • Major prion protein
      • ASCR
      • CD230
      • PrP
      • PrP27-30
      • PrP33-35C
      Background InformationMajor prion protein (UniProt P04156; also known as ASCR, CD230, PrP, PrP27-30, PrP33-35C) is encoded by the PRNP (also known as ALTPRP, CJD, GSD, HDL1, KURU, PRIP, PRP, SENF) gene (Gene ID 5621) in human. The major prion protein (PrP) is synthesized with an N-terminal signal peptide (a.a. 1-22) and a C-terminal proptptide (a.a. 231-253) sequence, which are posttranslationally removed to yield the mature (a.a. 23-230) N-glycosylated, glycosylphosphatidylinositol (GPI)-anchored cell surface protein. Structurally, PrP consists of a long, disordered, flexible NH2-proximal region and a globular COOH-proximal domain linked together by a hydrophobic core (HC) region (a,a. 111-134) that is considered as a key region in both physiological and disease related processes involving the prion protein. The N-terminal region is composed of five octapeptide (PHGGGWGQ) repeats (a.a. 51-91) sandwiched between two positively charged clusters, CC1 & CC2 (a.a. 23-27 & 95-110). The exact physiological function of PrP is not known, although various biological functions have been suggested for this protein, including signal transduction, neurotransmitter metabolism, cell adhesion, antioxidant activity, neurogenesis, immune cell activation, copper metabolism and homeostasis of trace elements. Transmissible spongiform encephalopathies (TSEs) represent a family of rare and fatal neurodegenerative disorders, including Creutzfeldt-Jakob disease, Gerstmann-Sträussler-Scheinker syndrome, fatal familial insomnia and kuru in human, bovine spongiform encephalopathy in cattle, and scrapie in sheep. It is widely accepted that the conformational transition of the native and predominantly α-helical cellular PrP (PrPC) to a β-sheet-rich pathogenic scrapie isoform (PrPSc) is responsible for the accumulation of PrPSc aggregates.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Prion Protein Antibody, clone 12D6.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Prion Protein.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected prion protein in human cerebral cortex tissue sections.
      Biological Information
      ImmunogenHuman IgG1 Fc-tagged recombinant human prion protein internal fragment.
      EpitopeInternal (N-terminal half).
      Clone12D6.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 12D6.1 detects an epitope present in human PrP and the spliced isoform PrP(M8) reported by UniProt (P04156-1 & P04156-2), but absent in the alternative prion protein AltPrP (UniProt F7VJQ1-1).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRNP
      • ALTPRP
      • CJD
      • GSD
      • HDL1
      • KURU
      • PRIP
      • PRP
      • SENF
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight27.66/26.89 kDa (PrP/PrP(M8) prepro-form), 22.75/25.24 kDa (mature/pro-form) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting of recombinant human prion protein.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected 0.05 µg of human IgG1 Fc-tagged recombinant human prion protein internal fragment.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1186 04055977352986

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies