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MAB2226 Anti-Piccolo Antibody, clone 4G3.3

This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.

Anti-Piccolo Antibody, clone 4G3.3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Synapse & Synaptic Biology Gene Symbol: ACZ , Aczonin, DKFZp779G1236, KIAA0559 UniProt Number: Q9Y6V0
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MAB2226
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2226
      DescriptionAnti-Piccolo Antibody, clone 4G3.3
      Alternate Names
      • presynaptic cytomatrix protein
      • Aczonin
      Background InformationPiccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE].
      References
      Product Information
      FormatPurified
      Control
      • Rat brain lysate, mouse cerebellum
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin):
      Representative images from a previous lot.
      Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).
      Biological Information
      ImmunogenGST-tagged recombinant protein
      Clone4G3.3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes piccolo.
      IsotypeIgMκ
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse and Rat.
      Predicted to react with human based on 82% sequence homology and rat based on 92% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACZ
      • Aczonin
      • DKFZp779G1236
      • KIAA0559
      UniProt Number
      UniProt SummaryFUNCTION: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking (By similarity).
      SUBUNIT: Interacts with Rabac1/Pra1, RIMS2 and profilin (By similarity).
      SUBCELLULAR LOCATION: Cell junction, synapse (By similarity). Note=Concentrated at the presynaptic side of synaptic junctions (By similarity).
      ALTERNATIVE PRODUCTS: 3 named isoforms
      DOMAIN: C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
      SIMILARITY: Contains 2 C2 domains.
      SIMILARITY: Contains 1 PDZ (DHR) domain.
      SEQUENCE CAUTION:
      Sequence=AAD21789.2; Type=Erroneous gene model prediction;
      Molecular Weight547 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on rat brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2226 04053252727429

      Documentation

      Anti-Piccolo Antibody, clone 4G3.3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Piccolo Antibody, clone 4G3.3 Certificates of Analysis

      TitleLot Number
      Anti-Piccolo, Clone 4G3.3 - 4103094 4103094
      Anti-Piccolo, clone 4G3.3 - 2197285 2197285
      Anti-Piccolo, clone 4G3.3 - 2547196 2547196
      Anti-Piccolo, clone 4G3.3 - 3216025 3216025
      Anti-Piccolo, clone 4G3.3 - 3752785 3752785
      Anti-Piccolo, clone 4G3.3 - NG1545023 NG1545023
      Anti-Piccolo, clone 4G3.3 - NG1865038 NG1865038
      Anti-Piccolo, clone 4G3.3 -2607608 2607608
      Anti-Piccolo, clone 4G3.3 -2700988 2700988
      Anti-Piccolo, clone 4G3.3 Monoclonal Antibody 2955323
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      References

      Reference overviewPub Med ID
      IQ-ArfGEF/BRAG1 is associated with synaptic ribbons in the mouse retina.
      Osamu Katsumata,Naoki Ohara,Hideaki Tamaki,Tomoko Niimura,Hideaki Naganuma,Masahiko Watanabe,Hiroyuki Sakagami
      The European journal of neuroscience  30  2009

      Show Abstract       		19811534 19811534