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ABT59 Anti-Pericentrin Antibody

This Anti-Pericentrin Antibody is validated for use in WB, IC for the detection of Pericentrin.

Anti-Pericentrin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: PCNT, PCNT2 UniProt Number: O95613
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ABT59
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT59
      DescriptionAnti-Pericentrin Antibody
      Alternate Names
      • Pericentrin
      • Kendrin
      • Pericentrin-B
      Background InformationPericentrin binds to calmodulin and is expressed in the centrosome that serves as a multifunctional scaffold for anchoring proteins and protein complexes. Evidence suggests that pericentrin plays a role in microtubule nucleation and spindle organization. Recent studies show pericentrin plays a role in Majewski/micorcephalic osteodysplastic primordial dwarfism type II (MOPDII) and Seckel Syndrome. Recent use in network modeling indicates a direct link between breast cancer susceptibility to centrosome defects. Pericentrin is a potential marker for centrosome defects in cancer and cancer progression, as centrosomal abonormalities in solid tumors are accompanied by increased pericentrin levels and defects in pericentrin organization.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Pericentrin Antibody is validated for use in WB, IC for the detection of Pericentrin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Pericentrin in HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Pericentrin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human.
      Other homologies: Mouse (89% sequence homology). No homology to Rat was found.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. [provided by RefSeq].
      Gene Symbol
      • PCNT
      • PCNT2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM).

      SUBUNIT STRUCTURE: Interacts with CHD3. Interacts with CHD4; the interaction regulates centrosome integrity (By similarity). Interacts with DISC1 and PCM1. Binds calmodulin. Interacts with CDK5RAP2; the interation is leading to centrosomal localization of PCNT and CDK5RAP2.

      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton › centrosome. Note: Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.

      TISSUE SPECIFICITY: Expressed in all tissues tested, including placenta, liver, kidney and thymus.

      DOMAIN: Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.

      INVOLVEMENT IN DISEASE: Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2) [MIM:210720]; also known as osteodysplastic primordial dwarfism type 2. Adults with this rare inherited condition have an average height of 100 centimeters and a brain size comparable to that of a 3-month-old baby, but are of near-normal intelligence.

      SEQUENCE CAUTION: The sequence AAD10838.1 differs from that shown. Reason: Frameshift at position 3320.

      The sequence BAA23698.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

      The sequence BAC04252.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
      Molecular Weight~250 kDa observed.
      Uniprot describes 2 isoforms produced by alternative splicing with molecular weights at 378 & 355 kDa. However in endogenous lysates, Pericentrin is observed at ~250 kDa & ~150 kDa for western blots (Wen, Y., et al. (2008). J Biol Chem. 283(5):2793-2803).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Pericentrin in 10 µg of HeLa cell lysate. Western Blot was performed without Tween.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT59 04053252420634

      Documentation

      Anti-Pericentrin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Pericentrin Antibody Certificates of Analysis

      TitleLot Number
      Anti-Pericentrin - 2345062 2345062
      Anti-Pericentrin - 2385981 2385981
      Anti-Pericentrin - 2271194 2271194
      Anti-Pericentrin - 2293545 2293545
      Anti-Pericentrin - 3217087 3217087
      Anti-Pericentrin - 3460371 3460371
      Anti-Pericentrin - 3527767 3527767
      Anti-Pericentrin - 3723484 3723484
      Anti-Pericentrin - 4153071 4153071
      Anti-Pericentrin - NRG1916844 NRG1916844
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