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MABE1115-25UG Anti-Pax1 Antibody, clone 5A2

Detect Paired box protein Pax-1 using this rat monoclonal Anti-Pax1, clone 5A2, Cat. No. MABE1115-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Pax1 Antibody, clone 5A2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: Pax1, Pax-1 UniProt Number: P09084
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MABE1115-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIH(P), WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1115-25UG
      DescriptionAnti-Pax1 Antibody, clone 5A2
      Alternate Names
      • Paired box protein Pax-1
      Background InformationPaired box protein Pax-1 (UniProt: P09084) is encoded by the Pax1 (also known as Pax-1) gene (Gene ID: 18503) in murine species. Pax-1 is a transcriptional activator that plays a role in the formation of segmented structures of the embryo and has important role in the normal development of the vertebral column. Mutations in Pax1 gene are associated with vertebral malformations. Undulated homozygous mice exhibits vertebral malformations along the entire rostro-caudal axis. Pax genes are vital for the specification of progenitor cells and for maintenance of progenitor cell fate, including that of cancer cells. Pax1 gene is silenced by methylation in ovarian and cervical cancers and may serve as a tumor suppressor gene. The methylation levels of Pax1 gene is significantly higher in tumor tissues compared to non-tumor paracancerous tissues. Hence, methylation status of Pax1 may serve as useful biomarkers for the detection of cervical cancer and is also a good prognostic indicator for oral squamous cell carcinoma. (Ref.: Huang J et al (2017). Int. J. Environ. Res. Public Health. 14(2). pii: E216).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Paired box protein Pax-1 using this rat monoclonal Anti-Pax1, clone 5A2, Cat. No. MABE1115-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Pax1 in Western Blotting applications (Feederle, R., et. al. (2016). Monoclon Antib Immunodiagn Immunother. [Epub ahead of print]).

      Immunohistochemistry Analysis: A representative lot detected Pax1 in Immunohistochemistry applications (Feederle, R., et. al. (2016). Monoclon Antib Immunodiagn Immunother. [Epub ahead of print]; Kist, R., et. al. (2014). PLoS Genet. 10(10):e1004709).

      Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Pax1 in Embryonic mouse head (E14.5) (Courtesy of Dr. Heiko Peters, Ph.D., Institute of Genetic Medicine, Newcastle University, United Kingdom).

      Western Blotting Analysis: A 1:10 dilution from a representative lot detected Pax1 in d11.5 embryonic vertebral column tissue lysates (Courtesy of Dr. Heiko Peters, Ph.D., Institute of Genetic Medicine, Newcastle University, United Kingdom).
      Biological Information
      ImmunogenGST-tagged full-length murine recombinant paired box protein Pax-1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 5A2 detects Paired box protein Pax-1 in murine tissues.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteMouse, Human.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Pax1
      • Pax-1
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse esophagus tissue lysate.
      Western Blotting Analysis: 1 µg/mL of this antibody detected Pax1 in 10 µg of m ouse esophagus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1115-25UG 04054839437915