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MABC946 Anti-PKCζ Antibody, clone 14B12.3

Anti-PKCζ Antibody, clone 14B12.3 is an antibody against PKCζ for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-PKCζ Antibody, clone 14B12.3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: PRKCZ, PKC2 UniProt Number: Q05513
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MABC946
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC946
      DescriptionAnti-PKCζ Antibody, clone 14B12.3
      Alternate Names
      • Protein kinase C zeta type
      • nPKC-zeta
      • PKC-zeta
      • PKCζ
      Background InformationProtein kinase C zeta type (EC 2.7.11.13; UniProt Q05513; also known as nPKC-zeta, PKC-zeta) is encoded by the PRKCZ (also known as PKC2) gene (Gene ID 5590) in human. Protein kinase Cs (PKCs) are threonine/serine kinases that play important roles in cellular signal transductions. PKC isozymes are classified into three major classes based on their domain compositions and activator requirements. The conventional PKCs (cPKCs) include the alpha, beta I, beta II, and gamma isoforms. The novel PKCs (nPKCs) include the delta, epsilon, eta, and theta isoforms. Atypical PKCs (aPKCs) include the lambda (mouse ortholog to human PKC-lambda is called PKC-iota) and zeta isoforms. Both cPKCs and nPKCs bind diacylglycerol (DAG) via their C1 domain, while only cPKCs, but not nPKCs bind Ca+2 via their C2 domain. The aPKCs do not bind either DAG or calcium for activity. All three classes of PKCs contain a pseudosubstrate sequence in their N-terminal region that keeps the substrate-binding cavity occupied via intramolecular interaction when the enzymes are in their inactive conformation. Conformation change upon enzyme activation frees the substrate-binding site.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PKCζ Antibody, clone 14B12.3 is an antibody against PKCζ for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected PRKCZ in 10 µg of PC12 cell lysate.
      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PRKCZ in human cerebral cortex, human cerebellum and mouse brain tissues.
      Biological Information
      ImmunogenGST-tagged recombinant human PKCζ C-terminal fragment.
      EpitopeC-terminal region
      Clone4B12.3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with all three spliced isoforms of human PKC-zeta.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRKCZ
      • PKC2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~70 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected PKCζ in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC946 04055977301182