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ST1203 Anti-PGC-1α Mouse mAb (1F3.9)

This Anti-PGC-1α Mouse mAb (1F3.9) is validated for use in Immunoblotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation for the detection of PGC-1α.

Anti-PGC-1α Mouse mAb (1F3.9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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ST1203
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      OverviewRecognizes the ~113 kDa PGC-1α protein in COS cells.
      Catalogue NumberST1203
      Brand Family Calbiochem®
      Application Data
      Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: DAB.

      Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: fluorescence.

      Detection of mouse PGC-1α by immunoblotting. Sample: Whole tissue extract from brown adipose tissue of mice held at room temperature (left 3 lanes) and mice held at 4°C for 5 hr (right three lanes). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesChang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
      Zhang, Y. et al. 2009. J. Biol. Chem. 284, 32813.
      Lai, L., et al. 2008. Genes Dev. 14, 1948.
      Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
      Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
      Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
      Product Information
      FormLiquid
      FormulationIn 50 mM PBS
      Positive controlBrown adipose tissue from mice exposed to cold
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunocytochemistry (5-10 µg/ml)
      Immunohistochemistry (5-10 µg/ml)
      Immunoprecipitation (1:100)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenA recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
      ImmunogenMouse
      Clone1F3.9
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Regulatory Review
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1203 0

      Documentation

      Anti-PGC-1α Mouse mAb (1F3.9) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PGC-1α Mouse mAb (1F3.9) Certificates of Analysis

      TitleLot Number
      ST1203

      References

      Reference overview
      Chang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
      Zhang, Y. et al. 2009. J. Biol. Chem. 284, 32813.
      Lai, L., et al. 2008. Genes Dev. 14, 1948.
      Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
      Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
      Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-February-2011 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Immunocytochemistry (5-10 µg/ml)
      Immunohistochemistry (5-10 µg/ml)
      Immunoprecipitation (1:100)
      Application Data
      Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: DAB.

      Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: fluorescence.

      Detection of mouse PGC-1α by immunoblotting. Sample: Whole tissue extract from brown adipose tissue of mice held at room temperature (left 3 lanes) and mice held at 4°C for 5 hr (right three lanes). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~113 kDa PGC-1α protein.
      BackgroundPGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors that regulates the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full length protein is detected at 113 kDa and is induced in BAT by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full length gene produces a 270 aa N-terminal splice variant that migrates at ~38 kDa and is induced by the same physiological signals which induce expression of the full length protein.
      HostMouse
      Immunogen speciesMouse
      ImmunogenA recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
      Clone1F3.9
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlBrown adipose tissue from mice exposed to cold
      FormLiquid
      FormulationIn 50 mM PBS
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Regulatory Review
      ReferencesChang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
      Zhang, Y. et al. 2009. J. Biol. Chem. 284, 32813.
      Lai, L., et al. 2008. Genes Dev. 14, 1948.
      Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
      Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
      Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.