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MABT533 Anti-PECAM-1 Antibody, clone 4A8.1

This mouse monoclonal Anti-PECAM-1 Antibody, clone 4A8.1, Cat. No. MABT533, is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of PECAM-1 (CD31).

Anti-PECAM-1 Antibody, clone 4A8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: PECAM1 UniProt Number: P16284
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MABT533
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT533
      Replaces04-1074
      DescriptionAnti-PECAM-1 Antibody, clone 4A8.1
      Alternate Names
      • Platelet endothelial cell adhesion molecule
      • CD31
      • EndoCAM
      • GPIIA
      • PECA1
      • PECAM-1
      Background InformationPlatelet endothelial cell adhesion molecule (UniProt P16284; also known as CD31, EndoCAM, GPIIA', PECA1, PECAM-1) is encoded by the PECAM1 gene (Gene ID 5175) in human. PECAM-1 (CD31) is a 130 kDa cell adhesion molecule that is expressed at the junctions of all continuous endothelia, as well as most bone marrow-derived hematopoietic cells, including platelets, monocytes, granulocytes and some lymphocytes. The human PECAM1 and murine Pecam1 genes both produce multiple spliced isoforms with varying lengths in cytoplasmic domain, with the isoform Long being the major form expressed in human tissues and endothelial cells (ECs), and isoform delta 14-15 being the predominantly expressed murine form. PECAM-1 plays an import role in maintaining vascular integrity and function by mediating ECs interactions with pericytes and smooth muscle cells (SMCs). PECAM-1 is initially produced with a signal peptide (human a.a. 1-27, murine a.a. 1-17) sequence, the removal of which yields the mature protein with a large extracellular region (human a.a. 28-601, mouse a.a. 18-590) composed of six C2-type Ig-like domains, followed by a transmembrane seqment (human a.a. 602-620, mouse a.a. 591-609) and a cytoplasmic tail (human a.a. 621-738, mouse a.a. 610-727) that plays an essential role in PECAM-1-mediated cellular signaling.
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-PECAM-1 Antibody, clone 4A8.1, Cat. No. MABT533, is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of PECAM-1 (CD31).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PECAM-1 in human cerebellum, lung, and pancreas tissue sections.

      Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PECAM-1 in 10 µg of Jurkat cell lysate.
      Biological Information
      ImmunogenGST-tagged recombinant human PECAM-1 cytoplasmic domain.
      Epitopecytoplasmic domain
      Clone4A8.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4A8.1 was raised against the cytoplasmic domain of PECAM-1 (CD31) isoform Long. Reactivity toward spliced isoforms Delta12, Delta13, Delta14, Delta14-15, and Delta15 with various cytomplasmic domain deletions is possible, but has not not been confirmed.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PECAM1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~130 kDa observed. Target band size appears larger than the calculated molecular weights of 79.58/77.52/77.25/77.46/76.30/78.42 kDa (mature isoform Long/Delta12/Delta13/Delta14/Delta14-15/Delta15) and 82.54/80.48/80.21/80.42/79.26/81.38 kDa (isoform Long/Delta12/Delta13/Delta14/Delta14-15/Delta15 pro-form) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in THP-1 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected PECAM-1 in 10 µg of THP-1 human acute monocytic leukemia cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABT533 04054839088926