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ST1643 Anti-PDK2 Mouse mAb (2G1)

This Anti-PDK2 Mouse mAb (2G1) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PDK2.

Anti-PDK2 Mouse mAb (2G1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Pyruvate Dehydrogenase Kinase 2

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ST1643
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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ST1643-100UG
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      Description
      OverviewRecognizes the ~40 kDa PDK2 protein in U-2 OS cells.
      Catalogue NumberST1643
      Brand Family Calbiochem®
      SynonymsAnti-Pyruvate Dehydrogenase Kinase 2
      Application Data
      Detection of human PDK2 by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human PDK2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (0.8 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlU-2 OS cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (0.8 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
      Clone2G1
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1643-100UG 04055977223781

      Documentation

      Anti-PDK2 Mouse mAb (2G1) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-Pyruvate Dehydrogenase Kinase 2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (0.8 µg/ml)
      Application Data
      Detection of human PDK2 by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human PDK2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (0.8 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~40 kDa PDK2 protein.
      BackgroundThe PDK2 protein inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 α subunit, thus contributing to the regulation of glucose metabolism.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
      Clone2G1
      IsotypeIgG2a
      Specieshuman
      Positive controlU-2 OS cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review