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PC376 Anti-P2X4 Receptor (373-388) Rabbit pAb

Anti-P2X4 Receptor (373-388) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC376
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~65 kDa P2X4 receptor protein in mouse brain.
      Catalogue NumberPC376
      Brand Family Calbiochem®
      Application Data
      Detection of mouse P2X4 receptor by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-P2X4 Receptor (373-388) Rabbit pAb (Cat. No. PC376) (2 µg/ml) pre-incubated without peptide (lane A) or with peptide control (25 µg/ml, lane B). Detection: chemiluminescence.

      Detection of mouse P2X4 receptor by staining floating sections. Sample: Mouse cortex tissue fixed with 4% paraformaldehyde. Primary antibody: Anti-P2X4 Receptor (373-388) Rabbit pAb (Cat. No. PC376) (2 µg/ml). Detection: DAB
      References
      ReferencesLe, K.-T., et al. 1998. Neuroscience 83, 177.
      Nicke, A., et al. 1998. EMBO J. 17, 3016.
      Ralevic, V. and Burnstock, G. 1998. Pharmcolol. Rev. 50, 413.
      North, R.A. and Barnard, E.A. 1997. Curr. Opin. Neurobiol. 7, 346.
      Radford, K.M., et al. 1997. J. Neurosci. 17, 6529.
      Buell, G., et al. 1996. EMBO J. 15, 55.
      Collo, G., et al. 1996. J. Neurosci. 16, 2495.
      Seguela, P., et al. 1996. J. Neurosci. 16, 448.
      Bo, X., et al. 1995. FEBS Lett. 375, 129.
      Lewis, C., et al. 1995. Nature 377, 432.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlMouse brain, rat brain, or SK-N-SH cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesFrozen Sections (1-2.5 µg/ml)
      Immunoblotting (5 µg/ml)
      Application CommentsA protein database search confirms that this peptide sequence is unique to the P2X4 receptor protein. The signal was completely abolished in immunoblotting by preincubating the purified antibody with blocking peptide at 25 µg/ml. Immunohistochemistry was performed on floating mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M (approximately 2 µg/ml; data not shown). Immunohistochemical staining results correlate well with other independent reports and in situ hybridization studies. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (KYVEDYEQGLSGEMNQ) corresponding to amino acids 373-388 of rat P2X4 receptor, conjugated to KLH
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC376 0

      Documentation

      Anti-P2X4 Receptor (373-388) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-P2X4 Receptor (373-388) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC376

      References

      Reference overview
      Le, K.-T., et al. 1998. Neuroscience 83, 177.
      Nicke, A., et al. 1998. EMBO J. 17, 3016.
      Ralevic, V. and Burnstock, G. 1998. Pharmcolol. Rev. 50, 413.
      North, R.A. and Barnard, E.A. 1997. Curr. Opin. Neurobiol. 7, 346.
      Radford, K.M., et al. 1997. J. Neurosci. 17, 6529.
      Buell, G., et al. 1996. EMBO J. 15, 55.
      Collo, G., et al. 1996. J. Neurosci. 16, 2495.
      Seguela, P., et al. 1996. J. Neurosci. 16, 448.
      Bo, X., et al. 1995. FEBS Lett. 375, 129.
      Lewis, C., et al. 1995. Nature 377, 432.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-January-2009 JSW
      ApplicationFrozen Sections (1-2.5 µg/ml)
      Immunoblotting (5 µg/ml)
      Application Data
      Detection of mouse P2X4 receptor by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-P2X4 Receptor (373-388) Rabbit pAb (Cat. No. PC376) (2 µg/ml) pre-incubated without peptide (lane A) or with peptide control (25 µg/ml, lane B). Detection: chemiluminescence.

      Detection of mouse P2X4 receptor by staining floating sections. Sample: Mouse cortex tissue fixed with 4% paraformaldehyde. Primary antibody: Anti-P2X4 Receptor (373-388) Rabbit pAb (Cat. No. PC376) (2 µg/ml). Detection: DAB
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~65 kDa P2X4 receptor protein.
      BackgroundExtracellular ATP acts as a signaling molecule to mediate a wide variety of physiological responses through interactions with the P2 family of receptors. P2 receptors have been classified into two groups, the P2X family of ligand-gated ion channels, and the P2Y family of metabotropic G-protein coupled receptors. To date, seven P2X receptor subtypes and eight different P2Y receptor subtypes have been identified. The P2X receptor subunits exhibit approximately 35%-48% identity with each other at the amino acid level, and have been shown to form both homo- and hetereromultimeric complexes. The various P2X receptor subunits exhibit a broad pattern of distribution, being localized to different types of immune cells, glands, smooth muscle, skeletal muscle, and the central and peripheral nervous systems. The P2X4 receptor subunit is predominantly expressed in the central nervous system and has a widespread distribution as determined by in situ hybridization and immunohistochemistry.
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide (KYVEDYEQGLSGEMNQ) corresponding to amino acids 373-388 of rat P2X4 receptor, conjugated to KLH
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlMouse brain, rat brain, or SK-N-SH cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsA protein database search confirms that this peptide sequence is unique to the P2X4 receptor protein. The signal was completely abolished in immunoblotting by preincubating the purified antibody with blocking peptide at 25 µg/ml. Immunohistochemistry was performed on floating mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M (approximately 2 µg/ml; data not shown). Immunohistochemical staining results correlate well with other independent reports and in situ hybridization studies. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesLe, K.-T., et al. 1998. Neuroscience 83, 177.
      Nicke, A., et al. 1998. EMBO J. 17, 3016.
      Ralevic, V. and Burnstock, G. 1998. Pharmcolol. Rev. 50, 413.
      North, R.A. and Barnard, E.A. 1997. Curr. Opin. Neurobiol. 7, 346.
      Radford, K.M., et al. 1997. J. Neurosci. 17, 6529.
      Buell, G., et al. 1996. EMBO J. 15, 55.
      Collo, G., et al. 1996. J. Neurosci. 16, 2495.
      Seguela, P., et al. 1996. J. Neurosci. 16, 448.
      Bo, X., et al. 1995. FEBS Lett. 375, 129.
      Lewis, C., et al. 1995. Nature 377, 432.

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