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PC376 Anti-P2X4 Receptor (373-388) Rabbit pAb

Anti-P2X4 Receptor (373-388) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC376
  
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      Overview

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~65 kDa P2X4 receptor protein in mouse brain.
      Catalogue NumberPC376
      Brand Family Calbiochem®
      Application Data
      Detection of mouse P2X4 receptor by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-P2X4 Receptor (373-388) Rabbit pAb (Cat. No. PC376) (2 µg/ml) pre-incubated without peptide (lane A) or with peptide control (25 µg/ml, lane B). Detection: chemiluminescence.

      Detection of mouse P2X4 receptor by staining floating sections. Sample: Mouse cortex tissue fixed with 4% paraformaldehyde. Primary antibody: Anti-P2X4 Receptor (373-388) Rabbit pAb (Cat. No. PC376) (2 µg/ml). Detection: DAB
      References
      ReferencesLe, K.-T., et al. 1998. Neuroscience 83, 177.
      Nicke, A., et al. 1998. EMBO J. 17, 3016.
      Ralevic, V. and Burnstock, G. 1998. Pharmcolol. Rev. 50, 413.
      North, R.A. and Barnard, E.A. 1997. Curr. Opin. Neurobiol. 7, 346.
      Radford, K.M., et al. 1997. J. Neurosci. 17, 6529.
      Buell, G., et al. 1996. EMBO J. 15, 55.
      Collo, G., et al. 1996. J. Neurosci. 16, 2495.
      Seguela, P., et al. 1996. J. Neurosci. 16, 448.
      Bo, X., et al. 1995. FEBS Lett. 375, 129.
      Lewis, C., et al. 1995. Nature 377, 432.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlMouse brain, rat brain, or SK-N-SH cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesFrozen Sections (1-2.5 µg/ml)
      Immunoblotting (5 µg/ml)
      Application CommentsA protein database search confirms that this peptide sequence is unique to the P2X4 receptor protein. The signal was completely abolished in immunoblotting by preincubating the purified antibody with blocking peptide at 25 µg/ml. Immunohistochemistry was performed on floating mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M (approximately 2 µg/ml; data not shown). Immunohistochemical staining results correlate well with other independent reports and in situ hybridization studies. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (KYVEDYEQGLSGEMNQ) corresponding to amino acids 373-388 of rat P2X4 receptor, conjugated to KLH
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      PC376 0