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PC362 Anti-Orexin A (14-33) (Ab-2) Rabbit pAb

Anti-Orexin A (14-33) (Ab-2) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC362
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      B, H, M, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes orexin A in mouse and rat brain. Supplied with a control peptide.
      Catalogue NumberPC362
      Brand Family Calbiochem®
      Application Data
      Detection of human Orexin A by staining free-floating sections. Sample: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Orexin A (14-33) (Ab-2) Rabbit pAb (Cat. No. PC362) (1:3500).
      References
      ReferencesDe Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
      Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
      Sakurai, T., et al. 1998. Cell 92, 573.
      Schwartz, M.W. 1998. Nat. Med. 4, 385.
      Product Information
      FormLiquid
      FormulationSerum or ascites diluted in 5 mM phosphate buffer, pH 7.5 and 25 µg lyophilized control.
      Positive controlMouse or rat brain
      Preservative≤0.1% sodium azide (antibody only)
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Free-floating Sections
      Frozen Sections
      Immunofluorescence
      Application NotesELISA (see comments)
      Free-floating Sections (1:3500)
      Frozen Sections (1:3500)
      Immunofluorescence (1:2000)
      Application CommentsImmunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the antiserum with control peptide at 10-6M. Staining is observed in the lateral hyothalamus. Unconjugated Orexin A Control Peptide has been included for use as an internal control for antibody specificity. This antibody has been tested to work in ELISA. Also available in purified form, Anti-Orexin A (14-33) (Ab-1) Rabbit pAb, Cat. No. PC345. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw of antibody, aliquot and freeze (-20°C). Reconstitute control peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC362 0

      Documentation

      Anti-Orexin A (14-33) (Ab-2) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Orexin A (14-33) (Ab-2) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC362

      References

      Reference overview
      De Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
      Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
      Sakurai, T., et al. 1998. Cell 92, 573.
      Schwartz, M.W. 1998. Nat. Med. 4, 385.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      ApplicationELISA (see comments)
      Free-floating Sections (1:3500)
      Frozen Sections (1:3500)
      Immunofluorescence (1:2000)
      Application Data
      Detection of human Orexin A by staining free-floating sections. Sample: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Orexin A (14-33) (Ab-2) Rabbit pAb (Cat. No. PC362) (1:3500).
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes orexin A. Supplied with a control peptide.
      BackgroundThe hypothalamus has been shown to play a central role in the regulation of energy homeostasis and in the control of feeding behavior. Two homologous peptides have recently been identified for which the message and protein have been localized to the lateral hypothalamus. Orexin A (hypocretin 1), a 33 amino acid peptide, and orexin B (hypocretin 2), a 28 amino acid peptide, are both encoded by a single mRNA transcript. These peptides stimulate food consumption in a dose-dependent manner when administered intracerebroventricularly, and precursor mRNA levels are upregulated upon fasting. In addition to the orexins (hypocretins), two G-protein coupled receptors, designated orexin receptor-1 and orexin receptor-2, have been identified which presumably mediate the signaling events from these neuropeptides. Competitive radioligand binding assays suggest that the orexin receptor-1 is selective for orexin A, while orexin receptor-2 is nonselective for both peptides.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
      IsotypeIgG
      Speciesbovine, human, mouse, rat
      Positive controlMouse or rat brain
      FormLiquid
      FormulationSerum or ascites diluted in 5 mM phosphate buffer, pH 7.5 and 25 µg lyophilized control.
      Preservative≤0.1% sodium azide (antibody only)
      CommentsImmunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the antiserum with control peptide at 10-6M. Staining is observed in the lateral hyothalamus. Unconjugated Orexin A Control Peptide has been included for use as an internal control for antibody specificity. This antibody has been tested to work in ELISA. Also available in purified form, Anti-Orexin A (14-33) (Ab-1) Rabbit pAb, Cat. No. PC345. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw of antibody, aliquot and freeze (-20°C). Reconstitute control peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesDe Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
      Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
      Sakurai, T., et al. 1998. Cell 92, 573.
      Schwartz, M.W. 1998. Nat. Med. 4, 385.