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MAB1580-I-25UL Anti-Oligodendrocytes Antibody, clone NS-1

MAB1580-I-25UL
25 µL  
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      Overview

      Description
      Catalogue NumberMAB1580-I-25UL
      DescriptionAnti-Oligodendrocytes Antibody, clone NS-1
      Background InformationOligodendrocytes large glial cells found in the central nervous system. They are responsible for production of myelin sheath that insulates neuronal axons. The first step in the generation of oligodendrocytes is the emergence of oligodendrocyte precursors (OPCs) from embryonic radial glia. Subsequently, OPCs differentiate into immature oligodendrocytes before they begin to generate myelin. This process is regulated by both intrinsic and extrinsic signals in cells. Oligodendrocytes have only a brief period for myelination early during differentiation, and mature oligodendrocytes are incapable of myelinating. An essential signal for the onset of myelination is reported to be provided by the electrical activity of neurons. Although the ATP released from axons firing axon potentials does not directly act on OPCs or oligodendrocytes, it triggers the release of leukemia inhibitory factor (LIF) from astrocytes, which promotes myelination. Although the electrical activity of neurons is an essential promyelinating factor, other factors also come into play in this process. During development, all growing nerve fibers are shown to express the polysialylated-neural cell adhesion molecule (PSA-NCAM) that can prevent homophilic NCAM NCAM adhesion and cell-cell interactions. When this form of NCAM is downregulated, as it is in electrically active neurons, only then the process of myelination can proceed. (Ref.: Bradl, M., and Lassmann, H. (2010). Acta Neuropathol. 119(1); 37-53; Berto, S., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(48); 24334-24342).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Oligodendrocytes, clone NS-1, Cat. No. MAB1580-I, is a mouse monoclonal antibody that detects Oligodendrocytes and is tested for use in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Applications: A representative lot detected Oligodendrocytes in Immunohistochemistry applications (Luna-Sanchez, M., et. al. (2015). EMBO Mol Med. 7(5):670-87; Jendresen, C.B., et. al. (2015). J Biol Chem. 290(8):5053-64).

      Immunocytochemistry Analysis: A representative lot detected Oligodendrocytes in Immunocytochemistry applications (Jiao, Q., et. al. (2016). Mol Med Rep. 14(5):4599-4605).

      Immunofluorescence Analysis: A representative lot detected Oligodendrocytes in Immunofluorescence applications (Kenzo, Uchida, et. al. (2012). Eur Spine J. 21(3):490-7).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRat olfactory bulb cells.
      EpitopeUnknown
      CloneNS-1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone NS-1 is a mouse monoclonal antibody that detects Oligodendrocytes and their myelin sheaths. It stains both early and mature oligodendrocytes.
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Human, Hamster, Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in Oligodendrocyte precursor cells.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected in Oligodendrocyte precursor cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1580-I-25UL 04065265237819