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CB1045 Anti-OCLN Mouse pAb

This Anti-OCLN Mouse pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of OCLN.

Anti-OCLN Mouse pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Occludin

CB1045
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, MMPolyclonal Antibody
      Description
      OverviewRecognizes the ~55-60 kDa OCLN protein in HeLa cells and human and mouse liver tissue.
      Catalogue NumberCB1045
      Brand Family Calbiochem®
      SynonymsAnti-Occludin
      Application Data
      Detection of human OCLN by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse OCLN by immunoblotting. Sample: Whole tissue extract from mouse liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human OCLN by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1:1200) and an anti-CSNK1E mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHuman liver tissue, Mouse liver tissue, HeLa cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunocytochemistry (1:50-1:1200)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, human OCLN (aa 1-522)
      ImmunogenHuman
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CB1045 0

      Documentation

      Anti-OCLN Mouse pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Occludin
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunocytochemistry (1:50-1:1200)
      Application Data
      Detection of human OCLN by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse OCLN by immunoblotting. Sample: Whole tissue extract from mouse liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human OCLN by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1:1200) and an anti-CSNK1E mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
      DescriptionPurifried rabbit polyclonal antibody. Recognizes the ~55-60 kDa OCLN protein.
      BackgroundOCLN is an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described.
      HostMouse
      Immunogen speciesHuman
      ImmunogenFull-length, human OCLN (aa 1-522)
      IsotypeIgG
      Specieshuman, mouse
      Positive controlHuman liver tissue, Mouse liver tissue, HeLa cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review