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MAB1277-I-100UG Anti-Nucleoli Antibody, clone 125-10

Anti-Nucleoli, clone 125-10, Cat. No. MAB1277-I, is a mouse monoclonal antibody that detects nucleoli and is tested for use in Immunofluorescence and Immunocytochemistry.

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MAB1277-I-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB1277-I-100UG
      DescriptionAnti-Nucleoli Antibody, clone 125-10
      Alternate Names
      • Nucleolus
      Background InformationThe nucleoli small basophilic, spherical, non-membrane enclosed, highly conserved, structures that are localized within the nucleus. Usually, they are localized in the central nuclear region or close to the nuclear membrane. They are formed around nucleolus organizer regions (NORs) consisting of ribosomal DNA (rDNA). They are structurally organized into the fibrillar center, the dense fibrillar component, and the granular component region. Clone 125-10 detects human nucleoli by immunocytochemistry.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Nucleoli, clone 125-10, Cat. No. MAB1277-I, is a mouse monoclonal antibody that detects nucleoli and is tested for use in Immunofluorescence and Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesTested Applications

      Immunocytochemistry Analysis: A representative lot detected Nucleoli in Immunocytochemistry applications (Endo, A., et al. (2009). J Biol Chem. 284(41):27918-27923; Endo, A., et al. (2009). J Cell Sci. 122(Pt 5):678-86).

      Immunofluorescence Analysis: A representative lot detected Nucleoli in Immunofluorescence applications (Clements, D., et al. (2007). Am J Physiol Lung Cell Mol Physiol. 292(1):L258-66; Vallee, D., et al. (2004). J Med Genet. 41(10):778-83; Ohno, M., et al. (2002). Chromosoma. 111(3):201-13).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRaji cell nuclei.
      EpitopeUnknown
      Clone125-10
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 125-10 is a mouse monoclonal antibody that specifically detects nucleoli in human cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Nucleoli in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1277-I-100UG 04065267404066