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487924 Anti-Nitrotyrosine Rabbit pAb

Anti-Nitrotyrosine Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
487924
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes nitrotyrosine.
      Catalogue Number487924
      Brand Family Calbiochem®
      Application Data
      Detection of bovine nitrotyrosine by immunoblotting. Samples: Low molecular weight standards (lane 1), BSA (lane 2), peroxynitrite-treated BSA (lane 3 and 4), peroxynitrite-treated cell lysate (lane 5). Primary antibody: Anti-Nitrotyrosine Rabbit pAb (Cat. No. 487924) (Assay Dependent on Primary Anitbody). Detection: chemiluminescence.
      References
      ReferencesMyatt, L., et al. 1996. Hypertension 28, 488.
      Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.
      Product Information
      FormLiquid
      FormulationIn TBS, 0.5 mg/ml BSA, 50% glycerol, pH 7.4.
      Negative controlBSA (untreated)
      Positive controlCells treated with peroxynitrite
      Preservative≤0.1% sodium azide
      ReconstitutionReconstitute with 500 μl H₂O.
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-2 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenperoxynitrite-treated KLH
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      487924 0

      Documentation

      Anti-Nitrotyrosine Rabbit pAb Certificates of Analysis

      TitleLot Number
      487924

      References

      Reference overview
      Myatt, L., et al. 1996. Hypertension 28, 488.
      Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2007 RFH
      ApplicationImmunoblotting (1-2 µg/ml)
      Application Data
      Detection of bovine nitrotyrosine by immunoblotting. Samples: Low molecular weight standards (lane 1), BSA (lane 2), peroxynitrite-treated BSA (lane 3 and 4), peroxynitrite-treated cell lysate (lane 5). Primary antibody: Anti-Nitrotyrosine Rabbit pAb (Cat. No. 487924) (Assay Dependent on Primary Anitbody). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes nitrotyrosine-containing proteins.
      BackgroundNitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by Nitric oxide synthase. The reaction of NO with superoxide which is often present under conditions of oxidative stress, forms peroxynitrite, a strong oxidant which can nitrosate tyrosine residues on proteins. Nitrotyrosine has been shown to be present in proteins from artherosclerotic lesions of human carotid arteries and also in placenta during pre-eclampsia.
      HostRabbit
      Immunogenperoxynitrite-treated KLH
      IsotypeIgG
      Speciesa broad range of species
      Positive controlCells treated with peroxynitrite
      Negative controlBSA (untreated)
      FormLiquid
      FormulationIn TBS, 0.5 mg/ml BSA, 50% glycerol, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMyatt, L., et al. 1996. Hypertension 28, 488.
      Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.