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MAB353A4 Anti-Nestin Antibody, clone rat-401, Alexa Fluor® 488 conjugate

Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research, Neuroscience Research Sub-Category: Neurofilament & Neuron Metabolism, Neuronal & Glial Markers Gene Symbol: NES, Nbla00170, FLJ21841 UniProt Number: P21263
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MAB353A4
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberMAB353A4
      DescriptionAnti-Nestin Antibody, clone rat-401, Alexa Fluor® 488 conjugate
      Alternate Names
      • rat401
      • NES
      • Nbla00170
      • FLJ21841
      Background InformationNestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Mouse cortical neural stem cells (SCR029)
      PresentationPurified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.
      Quality LevelMQ100
      Applications
      ApplicationDetect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.
      Key Applications
      • Immunocytochemistry
      Application NotesEvaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).
      Biological Information
      ImmunogenEmbryonic rat spinal cord
      Clonerat-401
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      Specificity
      This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Mouse and Rat. Antibody is not reactive with human Nestin protein.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NES
      • Nbla00170
      • FLJ21841
      Purification MethodProtein G Purified
      UniProt Number
      Molecular WeightThe unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDA.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB353A4 04053252597763

      Documentation

      Anti-Nestin Antibody, clone rat-401, Alexa Fluor® 488 conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nestin Antibody, clone rat-401, Alexa Fluor® 488 conjugate Certificates of Analysis

      TitleLot Number
      Anti-Nestin, clone rat-401, Alexa -2592769 2592769
      Anti-Nestin, clone rat-401, Alexa -2649279 2649279
      Anti-Nestin, clone rat-401, Alexa -2665796 2665796
      Anti-Nestin, clone rat-401, Alexa Fluor#174; 488 conjugate - 2146015 2146015
      Anti-Nestin, clone rat-401, Alexa Fluor#174; 488 conjugate - 2088991 2088991
      Anti-Nestin, clone rat-401, Alexa Fluor#174; 488 conjugate - 2201527 2201527
      Anti-Nestin, clone rat-401, Alexa Fluor#174; 488 conjugate - Q2025114 Q2025114
      Anti-Nestin, clone rat-401, Alexa Fluor® 488 conjugate 3090517
      Anti-Nestin, clone rat-401, Alexa Fluor® 488 conjugate 2982449
      Anti-Nestin, clone rat-401, Alexa Fluor® 488 conjugate - 2394111 2394111
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies