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ABT331 Anti-Nephrin Antibody

Detect Nephrin using this rabbit polyclonal antibody, Anti-Nephrin Antibody validated for use in western blotting, IHC & Immunofluorescence.

Anti-Nephrin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: NPHS1 UniProt Number: O60500
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ABT331
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IHC, IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT331
      DescriptionAnti-Nephrin Antibody
      Alternate Names
      • Nephrin
      • NPHN
      • NPHS1
      • Renal glomerulus-specific cell adhesion receptor
      Background InformationNephrin (NPHS1, NPHN), also known as is Renal glomerulus-specific cell adhesion receptor, is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse kidney tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Nephrin using this rabbit polyclonal antibody, Anti-Nephrin Antibody validated for use in western blotting, IHC & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: 1 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.

      Immunofluorescence Analysis: 10 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Nephrin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Mouse
      Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human based on immunogen design.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • NPHS1
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~100 kDa observed. Uncharacterized bands may appear in some lysate(s). Despite its calculated molecular weight of 135 kDa, Nephrin often migrates at a lower than expected size in SDS-PAGE.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse kidney tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Nephrin in 15 µg of mouse kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT331 04053252923524

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies