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ABE2873 Anti-NUDT21

Anti-NUDT21, Cat. No. ABE2873, is a highly specific rabbit polyclonal antibody that targets Cleavage and polyadenylation specificity factor subunit 5 and has been tested in Immunoprecipitation and Western Blotting.

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ABE2873
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE2873
      DescriptionAnti-NUDT21
      Alternate Names
      • Cleavage and polyadenylation specificity factor subunit 5
      • Cleavage and polyadenylation specificity factor 25 kDa subunit
      • CPSF 25 kDa subunit
      • Nucleoside diphosphate-linked moiety X motif 21
      • Nudix motif 21
      • Pre-mRNA cleavage factor Im 25 kDa subunit
      Background InformationCleavage and polyadenylation specificity factor subunit 5 (UniProt: O43809; also known as Cleavage and polyadenylation specificity factor 25 kDa subunit, CFIm25, CPSF 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 25 kDa subunit) is encoded by the NUDT21 (also known as CFIM25, CPSF25, CPSF5) gene (Gene ID: 11051) in human. CFlm25 is a homodimeric protein that is a component of the cleavage factor Im (CFIm) complex, which plays a key role in pre-mRNA 3'-processing. It is involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. The interaction of CFlm25 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. It contains one Nudix hydrolase domain (aa 76-201). The region containing aa 2-147 is considered to be essential for RNA-binding. It can be acetylated by p300/CBP and acetylation results in a reduction in its interaction with Poly(A) polymerase alpha (PAPAO).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NUDT21, Cat. No. ABE2873, is a highly specific rabbit polyclonal antibody that targets Cleavage and polyadenylation specificity factor subunit 5 and has been tested in Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected NUDT21 in RNase A-treated HeLa whole-cell extract after IP (Katahira, J., et. al. (2013). Nucleic Acid Res. .41(14):7060-72).

      Immunoprecipitation Analysis: A representative lot detected NUDT21 in Co-immunoprecipitation with FLAG-tagged CF I cell lysates (Shimazu, T., et. al. (2007). J Biol Chem. 282(7):4470-8).
      Biological Information
      ImmunogenHis-tagged full length recombinant human Cleavage and polyadenylation specificity factor subunit 5 (CFIm25).
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Cleavage and polyadenylation specificity factor subunit 5 (CFIm25) in human cells.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NUDT21
      • CFIM25
      • CPSF25
      • CPSF5
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~25 kDa observed; 26.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Caco-2 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected NUDT21 in 10 µg of Caco-2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2873 04054839253157

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