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MABN797 Anti-NUB1 Antibody, clone 2E4.1

This Anti-NUB1 Antibody, clone 2E4.1 is validated for use in Western Blotting for the detection of NUB1.

Anti-NUB1 Antibody, clone 2E4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: NUB1, NYREN18 UniProt Number: Q9Y5A7
MABN797
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN797
      DescriptionAnti-NUB1 Antibody, clone 2E4.1
      Alternate Names
      • NEDD8 ultimate buster 1
      • NUB1
      • Negative regulator of ubiquitin-like proteins 1
      • Renal carcinoma antigen NY-REN-18
      Background InformationNEDD8 ultimate buster 1 (UniProt Q9Y5A7; also known as Negative regulator of ubiquitin-like proteins 1, Renal carcinoma antigen NY-REN-18) is encoded by the NUB1 (also known as BS4, NUB1L, NYREN18) gene (Gene ID 51667) in human. The Long (NUB1L) and Short spliced isoforms of NUB1 are interferon-inducible proteins that mediate the degradation of the ubiquitin-like protein NEDD8 and its protein conjugates. In addition, NUB1L can also facilitate the proteasomal degradation of another Ub-like protein FAT10. Cyclin E, p27, and p53 are among the proteins known to be targeted by NUB1. The ternary complex composed of UFD1, NPL4, and the AAA-ATPase P97/VCP (valosin-containing protein) plays a key role in recruiting and delivering ubiquitinated substrates to the proteasome. NUB1L is reported to interact with the complex and mediate NEDD8 proteasomal delivery.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-NUB1 Antibody, clone 2E4.1 is validated for use in Western Blotting for the detection of NUB1.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 2.0 µg/mL from a representative lot detected NUB1 in 10 µg of Mouse hypothalamus tissue, Rat hypocampus tissue, K562 cell, Molt-4 cell, and Raw 264.7 cell lysates .
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the C-terminal region of human NUB1.
      EpitopeC-terminal region
      Clone2E4.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with both isoform 1 (NUB1L) and isoform 2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NUB1
      • NYREN18
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~71 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected NUB1 in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN797 04055977298345