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MABC616 Anti-NOX4 Antibody, clone 10A8.1

This Anti-NOX4 Antibody, clone 10A8.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of NOX4.

Anti-NOX4 Antibody, clone 10A8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Toxicology & Drug Resistance Gene Symbol: NOX4, RENOX UniProt Number: Q9NPH5
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MABC616
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABC616
      DescriptionAnti-NOX4 Antibody, clone 10A8.1
      Alternate Names
      • NADPH oxidase 4
      • Kidney oxidase-1
      • Kidney superoxide-producing NADPH oxidase
      • KOX-1
      • Renal NAD(P)H-oxidase
      • NOX4
      Background InformationNADPH oxidase 4 (UniProt Q9NPH5; also known as Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX-1, Renal NAD(P)H-oxidase) is encoded by the NOX4 (also known as RENOX) gene (Gene ID 50507) in human. NOX4 belongs to the family of NADPH oxidases that modulate redox-dependent cellular signalling pathways via intracellular reactive oxygen species (ROS) generation. Seven family members have been identified in mammals (NOX1–5 and DUOX1/2) with member-specific cellular distribution, intracellular location, mechanisms of action and types of ROS produced. NOX4 produces predominantly H2O2, and is widely expressed in many cell types and adult tissues, including kidney, liver, heart and aorta. NOX4 plays an important role in homocysteine (Hcy) metabolism, which in turns is a determining factor that impacts on defence against environmental toxins. Nox-knocout in mice results in significantly decreased total plasma Hcy levels, causing markedly increased susceptibility to acetaminophen- (APAP-, paracetamol-) induced hepatoxicity as a result of significantly decreased cysteine production and hepatic GSH stores. Human NOX4 is a six-transmembrane (a.a. 17-37, 63-83, 104-124, 155-175, 189-209, 425-445) protein, having both its N- and C-terminal ends at the cytoplasmic side (a.a. 1-16 & 446-578).
      References
      Product Information
      FormatCulture Supernatant
      PresentationMouse monoclonal IgG2bκ hybridoma culture supernatant without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-NOX4 Antibody, clone 10A8.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of NOX4.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NOX4 in human kidney, pancreas, and testis tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to a fragment from the third extracellular loop of human NOX4.
      EpitopeThird extracellular loop.
      Clone10A8.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityImmunogen construct region is present in human NOX4 spliced isoform 1, 2 (Nox4A), and 8, missing in isoform 5 (Nox4C), 7, and 9, partially truncated in isoform 3 (Nox4E), 4 (Nox4D), and 6 (Nox4B) reported by UniProt (Q9NPH5).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOX4
      • RENOX
      Purification MethodUnpurified.
      UniProt Number
      Molecular Weight~67 kDa observed. 66.93 kDa (isoform 1), 58.41 kDa (isoform 2; Nox4A), 64.10 kDa (isoform 8), 27.63 kDa (isoform 3; Nox4E), 31.81 kDa (isoform 4; Nox4D), 62.75 kDa (isoform 6; Nox4B) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human kidney tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NOX4 in 10 µg of human kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC616 04055977355413

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies