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CA1029 Anti-N-Cadherin Mouse mAb (13A9)

CA1029
  
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      Overview

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~140 kDa N-cadherin protein in HeLa cells.
      Catalogue NumberCA1029
      Brand Family Calbiochem®
      Application Data
      Lane 1: Detection of human N-cadherin by immunoblotting. Sample: Whole cell lysate (25 µg) from HeLa cells. Primary antibody: Anti-N-Cadherin Mouse mAb (13A9) (Cat. No. CA1029) (1:1000). Detection: chemiluminescence. Lane 2: Detection of human N-cadherin by immunoprecipitation followed by immunoblotting. Antibody for immunoprecipitation: Anti N-Cadherin Mouse mAb (13A9) (Cat No. CA1029) (1:100/500 µg total protein). Immunoblotting conditions: same as lane 1.
      References
      ReferencesChristofori, G. 2006. Nature 44, 444.
      Cvallaro, U., et al. 2006. Exp. Cell Res. 312, 659.
      Cowin, P., et al. 2005. Curr. Op. Cell Biol. 17, 499.
      Product Information
      FormLiquid
      FormulationUndiluted ascites.
      Positive controlHeLa cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Immunoprecipitation (1:100)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant fusion protein consisting of the cytoplasmic domain of human N-cadherin, expressed in E. coli
      ImmunogenHuman
      Clone13A9
      HostMouse
      IsotypeIgG
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      CA1029 0