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CA1029 Anti-N-Cadherin Mouse mAb (13A9)

Anti-N-Cadherin Mouse mAb (13A9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CA1029
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~140 kDa N-cadherin protein in HeLa cells.
      Catalogue NumberCA1029
      Brand Family Calbiochem®
      Application Data
      Lane 1: Detection of human N-cadherin by immunoblotting. Sample: Whole cell lysate (25 µg) from HeLa cells. Primary antibody: Anti-N-Cadherin Mouse mAb (13A9) (Cat. No. CA1029) (1:1000). Detection: chemiluminescence. Lane 2: Detection of human N-cadherin by immunoprecipitation followed by immunoblotting. Antibody for immunoprecipitation: Anti N-Cadherin Mouse mAb (13A9) (Cat No. CA1029) (1:100/500 µg total protein). Immunoblotting conditions: same as lane 1.
      References
      ReferencesChristofori, G. 2006. Nature 44, 444.
      Cvallaro, U., et al. 2006. Exp. Cell Res. 312, 659.
      Cowin, P., et al. 2005. Curr. Op. Cell Biol. 17, 499.
      Product Information
      FormLiquid
      FormulationUndiluted ascites.
      Positive controlHeLa cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Immunoprecipitation (1:100)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant fusion protein consisting of the cytoplasmic domain of human N-cadherin, expressed in E. coli
      ImmunogenHuman
      Clone13A9
      HostMouse
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CA1029 0

      Documentation

      References

      Reference overview
      Christofori, G. 2006. Nature 44, 444.
      Cvallaro, U., et al. 2006. Exp. Cell Res. 312, 659.
      Cowin, P., et al. 2005. Curr. Op. Cell Biol. 17, 499.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      ApplicationImmunoblotting (1:1000)
      Immunoprecipitation (1:100)
      Application Data
      Lane 1: Detection of human N-cadherin by immunoblotting. Sample: Whole cell lysate (25 µg) from HeLa cells. Primary antibody: Anti-N-Cadherin Mouse mAb (13A9) (Cat. No. CA1029) (1:1000). Detection: chemiluminescence. Lane 2: Detection of human N-cadherin by immunoprecipitation followed by immunoblotting. Antibody for immunoprecipitation: Anti N-Cadherin Mouse mAb (13A9) (Cat No. CA1029) (1:100/500 µg total protein). Immunoblotting conditions: same as lane 1.
      DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~140 kDa N-cadherin protein.
      BackgroundCadherins are a large family of Ca2+-dependant cell adhesion transmembrane glycoproteins that play a role in cell to cell recognition. The classic cadherins include N-, R-, P-, B-, and E-cadherin. Although cadherins interact with similar intracellular proteins, they can exert specifc activities. Early non-invasive epithelial cells express E-cadherin. However, during the the process of epithelial to mesenchymal transition (EMT), the expression of E-cadherin is lost and the expression of N-cadherin is upregulated. This process, the cadherin switch, results in the transition of a benign tumor to a malignant and metastatic form. N-cadherin is a 140-kDa protein that has several functions that contribute to tumor invasion. It promotes cell-cell adhesion to N-cadherin stromal cells and it binds to and activates FGFRs that result in activation of the PI 3-K and MAPK pathways. N-cadherin is also cleaved by MMPs and a γ-secretase-like protease, releasing the extracellular domain of N-cadherin and the intracellular carboxy terminal.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant fusion protein consisting of the cytoplasmic domain of human N-cadherin, expressed in E. coli
      Clone13A9
      IsotypeIgG
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationUndiluted ascites.
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesChristofori, G. 2006. Nature 44, 444.
      Cvallaro, U., et al. 2006. Exp. Cell Res. 312, 659.
      Cowin, P., et al. 2005. Curr. Op. Cell Biol. 17, 499.