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MABF3160-100UL Anti-N-Cadherin Antibody, clone 8C11

Anti-N-Cadherin, clone 8C11, Cat. No. MABF3160, is a mouse monoclonal antibody that detects N-Cadherin and is tested for use in Flow Cytometry, Immunocytochemistry, Inhibition Assay, and Western Blotting.

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MABF3160-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3160-100UL
      DescriptionAnti-N-Cadherin Antibody, clone 8C11
      Alternate Names
      • Cadherin-2
      • CDw325
      • Neural Cadherin
      • CD325
      Background InformationCadherin-2 (UniProt: P19022; also known as CDw325, Neural cadherin, N-cadherin, CD325) is encoded by the CDH2 (also known as CDNH, NCAD) gene (Gene ID: 1000) in human. N-Cadherin is a single-pass type I membrane glycoprotein protein that plays a role in calcium-dependent cell adhesion. Its expression is observed in cells of the nervous tissue, vascular smooth muscle cells, and myocytes. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. N-cadherin are synthesized as precursor proteins with a signal peptide (aa 1-25) and a propeptide (aa 26-159) that are proteolytically cleaved to generate functional, mature protein. Newly synthesized proN-cadherin (aa 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. The mature N-Cadherin contains an extracellular domain (aa 160-724) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (aa 725-745), and a conserved cytoplasmic domain (aa 746-906) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis. A 69 amino acid segment of EC-4 of N-cadherin is reported to be essential and sufficient to increase cell motility and promote epithelial to mesenchymal transition. (Ref.: Kim, JB., et al. (2000). J. Cell Biol. 151(6); 1193-1205; Navarro, P., et al. (1998). J. Cell Biol. 140(6); 1475-1484).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-N-Cadherin, clone 8C11, Cat. No. MABF3160, is a mouse monoclonal antibody that detects N-Cadherin and is tested for use in Flow Cytometry, Immunocytochemistry, Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      • Inhibition
      Application NotesTested applications

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected N-Cadherin in Human brain and cerebellum tissue extracts.

      Western Blotting Analysis: A representative lot detected N-Cadherin in Western Blotting applications (Kim, J.B., et al. (2000). J Cell Biol. 151(6):1193-1206).

      Flow Cytometry Analysis: 1 µg from a representative lot detected N-Cadherin in one million KG-1 cells.

      Inhibition Assay: A representative lot inhibited motility of BT20 breast cancer cells (Kim, J.B., et al. (2000). J Cell Biol. 151(6):1193-1206).

      Flow Cytometry Analysis: A representative lot detected N-Cadherin in Flow Cytometry applications (Wahlig, S., et al. (2020). Sci Rep. 10(1):1681).

      Immunocytochemistry Analysis: A representative lot detected Cadherin-2 in Immunocytochemistry applications (Navarro, P., et al. (1998). J Cell Biol.;140(6):1475-1484).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenMBP-tagged recombinant fragment corresponding to 502 amino acids from the extracellular domain of human N-Cadherin.
      EpitopeExtracellular domain
      Clone8C11
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 8C11 is a mouse monoclonal antibody that detects N-Cadherin. It targets an epitope within the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Hamster
      Species Reactivity NoteHuman, Hamster
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHD2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~105 and 130 kDa observed; 99.81 and 97.04 kDa, respectively for isoforms 1 and 2. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human heart tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Cadherin-2 in Human heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3160-100UL 04065268900871