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MABC43-25UL Anti-McI-1 Antibody, clone RC13 (mouse monoclonal)

Detect Mcl-1 using this Anti-Mcl-1 Antibody, clone RC13 validated for use in WB, IH & IP.

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MABC43-25UL
25 µL  
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      Description
      Catalogue NumberMABC43-25UL
      DescriptionAnti-McI-1 Antibody, clone RC13 (mouse monoclonal)
      Alternate Names
      • myeloid cell leukemia sequence 1 (BCL2-related)
      • myeloid cell leukemia sequence 1, isoform 1
      • Bcl-2-like protein 3
      • Bcl-2-related protein EAT/mcl1
      • myeloid cell leukemia ES
      • induced myeloid leukemia cell differentiation protein Mcl-1
      Background InformationMcl-1 (Induced myeloid leukemia cell differentiation protein) is a potential transmembrane protein associated with the mitochondria belonging to the Bcl-2 family of proteins. Members of the Bcl-2 family have known participation in regulatory roles for apoptosis, cell growth, and differentiation. The hydrophobic carboxyl terminus is conserved among members of this family and in other members has proven to be a transmembrane region. Mcl-1 is found to be expressed early in myeloid differentiation. Two separate isoforms of Mcl-1 exist with opposing functions. Full length Mcl-1 (Isoform 1) promotes cell viability, over proliferation. Isoform 2 results from the skipping of the central exon of Mcl-1 during mRNA processing and the joining of the first and third exons. The function of the resulting protein induces apoptosis when accumulated. Pro-apoptotic function is shown to increase when isoform 1 and 2 dimerize.
      References
      Product Information
      FormatPurified
      Control
      • Raji cell lysate
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Mcl-1 using this Anti-Mcl-1 Antibody, clone RC13 validated for use in WB, IH & IP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A previous lot was used by an independent laboratory in follicular lymphoma, diffuse large cell lymphoma, and polymorphic lymphoma tissues. (Zhou, P., et al. (2001). Blood. 97(12):3902-3909).

      Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Han, J., et al. (2005). Biological Chemistry. 280:16383-16392).
      Biological Information
      ImmunogenRecombinant protein corresponding to human Mcl-1.
      CloneRC13
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq].
      Gene Symbol
      • MCL1
      • MCL1L
      • Mcl-1
      • mcl1/EAT
      • TM
      • MCL1S
      • EAT
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

      SUBUNIT STRUCTURE: Interacts with BAD, BOK, BIK and BFM By similarity. Interacts with PMAIP1. Isoform 1 interacts with BAX, BAK1, TPT1 and BCL2L11. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptototic BCL2-related proteins.

      SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein Potential. Cytoplasm. Mitochondrion. Nucleus › nucleoplasm. Note: Cytoplasmic, associated with mitochondria. INDUCTION: Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.

      PTM: Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.

      Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.

      Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.

      SEQUENCE SIMILARITIES: Belongs to the Bcl-2 family.
      Molecular Weight~37 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Raji cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected Mcl-1 in 10 µg of Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC43-25UL 04054839342837

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