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MABS1726 Anti-MPK-3 (DUSP6) Antibody, clone 5H6.1

Detect DUSP6 using this mouse monoclonal Anti-MPK-3 (DUSP6), clone 5H6.1 Antibody, Cat. No. MABS1726, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-MPK-3 (DUSP6) Antibody, clone 5H6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: DUSP6, HH19, MKP3, PYST1 UniProt Number: Q16828
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MABS1726
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1726
      DescriptionAnti-MPK-3 (DUSP6) Antibody, clone 5H6.1
      Alternate Names
      • Dual specificity protein phosphatase 6
      • Dual specificity protein phosphatase PYST1
      • MAP kinase phosphatase 3
      • Mitogen-activated protein kinase phosphatase 3
      • MKP-3
      Background InformationDual specificity protein phosphatase 6 (EC 3.1.3.16; EC 3.1.3.48; UniProt Q16828; also known as Dual specificity protein phosphatase PYST1, MAP kinase phosphatase 3, Mitogen-activated protein kinase phosphatase 3, MKP-3) is encoded by the DUSP6 (also known as HH19, MKP3, PYST1) gene (Gene ID 1848) in human. MPK-3 (DUSP6) belongs to the family of dual-specificity phosphatases (DUSPs) that mediate dephosphorylation of MAPKs on threonine/serine and tyrosine residues. DUSP6 is upregulated in HER2+ carcinomas and is among several DUSPs thought to be involved in breast cancer metastasis. DUSP1, DUSP4, and DUSP6 are induced during epithelial-to-mesenchymal transition (EMT) and are involved in forming and maintaining breast cancer stem cells (CSCs), DUSP1 knockdown reduces CSC formation, while DUSP4 or DUSP6 knockdown enhances CSC formation. PKC CK2alpha is reported to phosphorylate DUSP6 and increase its dephosphatase activity. ERK1/2, on the other hand, can phosphorylate DUSP6 to promote its proteosomal degradation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect DUSP6 using this mouse monoclonal Anti-MPK-3 (DUSP6), clone 5H6.1 Antibody, Cat. No. MABS1726, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MPK-3 (DUSP6) in human liver, pancreas, and tonsil tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human MPK-3 (DUSP6) rhodanese domain fragment.
      Clone5H6.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5H6.1 targets an epitope within the rhodanese domain present in both spliced isoforms of human MPK-3 (DUSP6) reported by UniProt (Q16828).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DUSP6
      • HH19
      • MKP3
      • PYST1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~42 kDa observed. 42.32 kDa (isoform 1) and 26.47 kDa (isoform 2; DUSP6-ALT) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected MPK-3 (DUSP6) in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1726 04054839088919