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ABN2282-100UG Anti-MINAR2/KIAA1024L

Anti-MINAR2/KIAA1024L, Cat. No. ABN2282, is a rabbit polyclonal antibody that detects MINAR2 and is tested for in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

ABN2282-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABN2282-100UG
      DescriptionAnti-MINAR2/KIAA1024L
      Alternate Names
      • Major intrinsically disordered NOTCH2-binding receptor 1-like
      • Membrane integral NOTCH2-associated receptor 2
      Background InformationMajor intrinsically disordered NOTCH2-binding receptor 1-like (UniProt: P59773; also known as Membrane integral NOTCH2-associated receptor 2) is encoded by the MINAR2 (also known as KIAA1024L) gene (Gene ID: 100127206) in human. MINAR2, a highly conserved, intrinsically disordered member of the MINAR family of proteins, has an extracellular domain, a single transmembrane domain, and a few amino acids in its cytoplasmic tail. Its expression is observed in the brain with high levels detected in midbrain, hypothalamus, and the pituitary gland. Loss of MINAR2 is reported to cause motor dysfunctions in murine models that resemble Parkinson's disease. MINAR2 knockout mice display significant alterations in their neuronal function, loss of TH positive neurons in pars compacta, and higher accumulation of -synuclein protein. It is reported to be localized to endoplasmic reticulum (ER) compartments and interacts with NOTCH2. ER stress, NOTCH folding, and its trafficking from the ER have been proposed to play a role in motor deficits associated with progression of Parkinson s disease. MINAR2 has also been linked to progressive hearing loss with alteration sin the stereocilia of hair cells. Loss of MINAR2 is shown to reduce cholesterol localization to the hair bundles in the ear that aggravates hair cell deficits. (Ref.: Bademci, G., et al. (2022) Proc. Natl. Acad. Sci. USA. 119(26); e2204084119; Ho, RX-Y., et al. (2020). Brain Commun.2(1); fcaa047).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-MINAR2/KIAA1024L, Cat. No. ABN2282, is a rabbit polyclonal antibody that detects MINAR2 and is tested for in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImmunohistochemistry Applications: A representative lot detected MINAR2/KIAA1024L in Immunohistochemistry application (Ho, R., et. al. (2020). Brain Commun. 2(1):fcaa047).

      Immunocytochemistry Analysis: A representative lot detected MINAR2/KIAA1024L in Immunocytochemistry applications (Ho, R., et. al. (2020). Brain Commun. 2(1):fcaa047).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenA linear peptide corresponding to 14 amino acids from the C-terminal half of human Membrane integral NOTCH2-associated receptor 2 (MINAR2).
      EpitopeC-terminal half
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Membrane integral NOTCH2-associated receptor 2 (MINAR2). It targets an epitope within 14 amino acids from the C-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Horse, Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MINAR2
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~22 kDa observe; 21.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from HEK293 cells transfected mouse MINAR2.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected mouse MINAR2 in lysates from HEK293 cells transfected mouse MINAR2, but not in lysates from untransfected cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN2282-100UG 04065269135234